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【摘要】目的:。方法: h,。用Western blot方法检测HMGB1在胞核及培养上清中的水平。结果: h后,培养上清中的HMGB1水平明显增加,胞核HMGB1含量减少,并存在剂量依赖关系。结论:,存在剂量依赖关系。
【关键词】 LPS HMGB1转位和释放
Abstract Objective:The purpose of this study was to investigate the translocation and release of HMGB1 in LPS-induced cell. Methods: cells were incubated with different concentrations LPS for 20 hours, the distribution and levels of HMGB1 in cells were subsequently analyzed by immunofluorence and Western blot,:HMGB1 accumulation in the culture media of LPS-induced cells was significantly increased, and the accumulation of HMGB1 in the nucleus was remarkably reduced. Conclusion: LPS can induce significant release of HMGB1 in cells in a dose-dependent manner.
Key words LPS; ce
lls; HMGB1 Translocation and release
以往研究表明,内毒素可刺激TNF-α、IL-1等早期细胞因子的合成和释放,被认为是动物多器官功能损害和死亡的核心因子。但抗TNF-α抗体治疗脓毒症的临床研究并未取得预期效果。新近研究发现,巨噬细胞可以产生一种新的细胞因子—高迁移率组蛋白B1(high mobility group box-1 protein,HMGB1)。该细胞因子具有释放晚、持续时间长的特点,被认为是引发机体炎症级联反应的重要介质。脂多糖(haride,LPS)是革兰氏阴性杆菌的主要致病成份,。
1 材料与方法
材料
;RPMI 1640培养基购自美国GIBCO公司;新生牛血清购自杭