文档介绍：五灵胶囊对LPS诱导枯否细胞释放细胞因子的影响
作者:王胜春,张英志,胡咏武,李晓玮,田卫斌
【关键词】,五灵胶囊
【Abstract】 AIM: To investigate the effects of WuLing Capsules on liver cell injuries induced by active molecules secreted by Kupffer’s cells. METHODS: Hepatocyte and Kupffer’s cells were isolated and purified from rat livers and then exposed to LPS and DGlan. The changes of hepatocytes and cytokines secreted from Kupffer’s cells were determined in the presence of WuLing Capsules. The protective effects of WuLing Capsule on hepatocyte injuries induced by LPS stimulation of kupffer’s cells were also examined. RESULTS: WuLing Capsules significantly reduced hepatocyte injuries induced by LPS and DGlan exposure and inhibited TNFα, IL6 and IL8 secretion of Kupffer’s cells. The hepatocyte injuries caused by active molecules released from Kupffer’s cells decreased in the presence of WuLing Capsule. CONCLUSION: WuLing Capsule may exert some therapeutic effects by the down regulation of cytokines in chronic hepatitis.
【Keywords】 WuLing capsules; Kupffer’s cells; cytokine; haride
【摘要】目的:探讨五灵胶囊对枯否细胞(Kupffer cells, KC)释放细胞因子介导肝细胞(hepatocellular cells, HC)损伤的作用. 方法:分离并纯化HC与KC并建立LPS及DGlaN损伤肝细胞模型,观察五灵胶囊对2种损伤模型的作用以及对LPS诱导KC释放细胞因子介导肝细胞损伤的作用. 结果:五灵胶囊2个剂量能有效地阻滞LPS,DGlaN损伤肝细胞以及降低LPS诱导KC释放TNFα,IL6,IL8水平,抑制KC释放细胞因子损伤肝细胞. 结论:五灵胶囊遏制KC大量释放细胞因子是治疗慢性肝炎作用机制之一.
【关键词】五灵胶囊; 枯否细胞; 细胞因子;脂多糖
0引言
慢性肝炎患者血清和肝组织中TNFα,IL6,IL8诱导肝损伤的发生和发展是与集聚在肝脏的KC密切相关,激活KC释放TNFα,IL6和IL8等炎性因子在肝脏免疫病理损害中起着重要作用[1],以往研究表明五灵胶囊对慢性肝炎患者良好的治疗作用已被临床和实验证实[2,3]. 五灵胶囊治疗肝损伤机制鲜见报道,我们采用大鼠肝细胞与KC细胞为靶细胞,在体外观察五灵胶囊(WuLing)对脂多糖(LPS)和D氨基半乳糖(DGlaN)诱导肝细胞损伤和LPS诱导KC释放因子的作用以及CD14表达,旨在阐明五灵胶囊降酶保肝的作用机制.
1材料和方法
①动物:SD大鼠,♂,[第四军医大学实验动物中心提供,动物合格证号:医动证字SC×K(军)200205];②试剂与药品: RPMI Medium 1640培养基(批号:1080385,USA. Gibco公司);DMEM・F12培养基(批号:1095578,);新生牛血清(NBS)(杭州四季青,批号:200206112,用前灭活56℃,30 min);脂多糖(haride, LPS)();丙氨酸氨基转移酶(alanine aminotransferase,ALT)、乳酸脱氢酶(Lactate dehydrogenase,LDHL)试剂盒(北京中生北控生物科技股份有限公司);单胺氧化酶(monoamine oxidase,MAO)、丙二醛(maleic dia