文档介绍：MTT比色法检测苦杏仁甙对兔晶状体上皮细胞增生的影响
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【摘要】目的研究苦杏仁甙(Amygdalin)对体外培养的兔晶状体上皮细胞增生的影响。方法取第2代对数生长期的兔晶状体上皮细胞,培养24h后,加入不同浓度的苦杏仁甙(、、、、、)作用24h后,采用MTT法观察苦杏仁甙对兔晶状体上皮细胞增生的影响。结果苦杏仁甙随着浓度的增加,兔晶状体上皮细胞增生的抑制作用逐渐增强。结论苦杏仁甙能有效抑制体外培养的兔晶状体上皮细胞增生,其抑制作用在一定浓度范围内有剂量依赖性。
【关键词】苦杏仁甙;兔晶状体上皮细胞;后囊浑浊;MTT比色法
[Abstract] Objective To study the effects of Amygdalin on the proliferation of rabbit lens epithelial cells(RLECs)in RLECs were primary the second generation of RLECs were incubated for 24 hours,we added different concentraions of Amygdalin for 24 cell proliferation of RLECs was determined by MTT The inhibitory rates of RLECs proliferation were increase
d in ways by Amygdalin in Amygdalin could effectively inhibit the proliferation of RLECs,and it might have broad prospects in the prevention of posterior capsular opacification.
[Key words] amygdalin;rabbit lens epithelial cells;posterior apsular opacification;MTT assay
后囊浑浊(posterior capsular opacification,PCO)是现代白内障囊外摘出人工晶状体植入术后最常见的并发症。后囊混浊的发生主要由于术后残留的晶状体上皮细胞(lens epithelial cells,LECs)发生增生及后囊的纤维化有关。其发生率往往与年龄相关,年龄越轻,发生率越高。在成人,术后发生率为30%~50%,在儿童则为100%。因此,如何有效地抑制晶状体上皮细胞增生对于PCO的防治具有重要意义。苦杏仁甙(Amygdalin)是中药桃仁的主要成分,是一种能生氰(cyanogensic)的化合物。存在于水果果核和坚果中。有研究表明它能有效抑制纤维母细胞增生[1],而且可以明显地抑制组织的纤维化[2]。这里我们采用MTT比色法研究其对体外培养