文档介绍：8BrcAMP联合顺铂作用于肺腺癌细胞A549的初步研究
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【摘要】目的:观察8溴环腺苷酸(8BrcAMP)联合顺铂(DDP)对人肺腺癌细胞A549生长及其表皮生长因子受体(EGFR)表达的影响。方法:MTT法测定不同浓度8BrcAMP、DDP以及两药联用处理人肺腺癌细胞A549后细胞增殖活性的差异;应用RTPCR技术检测EGFR mRNA表达水平的变化;Western blot技术检测EGFR蛋白表达水平的变化;利用流式细胞术测定细胞周期的改变。结果:8BrcAMP及DDP均能抑制人肺腺癌细胞A549的增殖,并呈浓度及时间依赖性;DDP联合8BrcAMP细胞抑制率显著高于相应浓度单药组(),两者呈现出相加的抗瘤效果。8BrcAMP可下调人肺腺癌A549细胞EGFR mRNA和蛋白的表达。8BrcAMP将细胞周期阻滞在G2/M期()。结论:8BrcAMP、DDP均可抑制A549细胞增殖。8BrcAMP可显著提高顺铂的细胞毒作用,增强化疗效果,并将细胞阻滞于G2/M期。8BrcAMP可以下调EGFR表达,可一定程度地逆转化癌细胞。
【关键词】 8溴环腺苷酸; 肺腺癌细胞A549; 表皮生长因子受体; 蛋白激酶A
[Abstract] Objective: To study the effect of 8
BrcAMP with cisplatin(DDP) on human pulmonary adenocarcinoma cell A549 growth and the expression level of EGFR in vitro. Methods: Proliferation ability and cell cycle changes of A549 cells were detected by MTT method and flow cytometry. The mRNA levels of EGFR were determined by RTPCR. The protein expression of EGFR were valued by Western blot. Results: Both DDP and 8BrcAMP could inhibit the proliferation of A549 cells in vitro by a timeand dosedependent manner. Compared with either 8BrcAMP or DDP alone, combining 8BrcAMP at 15 μmol·L-1 with DDP at μg·ml-1,1 μg·ml-1,2 μg·ml-1,4 μg·ml-1 respectively increased the growth inhibition rate of A549 cells significantly(). These results suggested additive actions of the two drugs. A549 cells showed G2/M stage arrest after 8BrcAMP administration(). Compared with the control group, expression of EGFR mRNA and EGFR protein significantly decreased in the 8BrcAMP group bining 8BrcAMP with DDP group. Conclusions: Both DDP and 8BrcAMP could inhibit the proliferation of lung cancer cells in vitro. 8BrcAMP has also shown synergistic antitum
or effects bined with DDP. 8BrcAMP can decrease the expression of EGFR and block the cell cycle at G2/M stage.
[Key words] 8BrcAMP; pulmonary adenocarcinma cell; epidermal growth factor receptor; protein kinases A
蛋白激