文档介绍：SARSCoV N蛋白和MAP19的相互作用
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【摘要】目的: 研究SARSCoV N蛋白与MAP19之间的相互作用。方法: 利用免疫共沉淀试验验证SARSCoV N蛋白与MAP19的相互作用, 并利用Western blot检测SARSCoV N蛋白对MAP19表达量的影响。结果: SARSCoV N蛋白与MAP19能够在细胞内相互作用, 且SARSCoV N能够显著提高MAP19的表达量。结论: SARSCoV N蛋白与MAP19能够在细胞内形成复合物, 将为进一步研究SARSCoV N蛋白与MAP19相互作用的生物学功能提供实验基础。
【关键词】 SARSCoV N; MAP19; 相互作用
[Abstract] AIM: Severe acute respiratory syndrome coronavirus (SARSCoV) is the etiological agent of SARS, an emerging disease characterized by atypical pneumonia. Using a yeast twohybrid screen with the nucleocapsid (N)protein of SARSCoV as a bait, the N protein was found to interact with MAP19, a nonenzymatic protein of MASP(mannan
associated serine protease). The interaction between SARSCoV N and MAP19 would be further tested in cells in this article. METHODS: The interaction between SARSCoV N and MAP19 was demonstrated by immuno coprecipitation, and the amount of MAP19 influenced by SARSCoV N was investgated by Western blot. RESULTS: The interaction between SARSCoV N and MAP19 was already demonstrated by immunocoprecipitation. SARSCoV N greatly increased the amount of MAP19. CONCLUSION: SARSCoV N can bind with MAP19 in cells. Our study may be conductive to further research into the molecular mechanism of action between SARSCoV N and MAP19.
[Keywords]SARSCoV N; MAP19; interaction
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