文档介绍：Survivin在胃腺癌、异型增生组织中的表达及其与p53、bcl-2的相关性
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【摘要】目的探讨凋亡抑制基因Survivin在胃癌、胃黏膜异型增生组织中的表达及其与突变型p53、bcl-2的相关性。方法应用免疫组织化学SP法检测50例胃腺癌组织、32例异型增生组织和20例正常胃黏膜组织中Survivin的表达。结果 Survivin在正常胃黏膜组织、胃癌组织及轻、中、重度异型增生组织中的阳性表达率分别为0%、56%、9%、10%、%,轻、中度异型增生组织中Survivin的阳性表达率明显低于重度异型增生和癌组织中的表达(),而在重度异型增生和癌组织间无显著性差异(); Survivin的表达与胃癌的组织分化、浆膜侵犯、淋巴结转移、临床分期呈正相关(),与年龄及肿瘤大小无相关性() 。结论(1)Survivin在胃癌及异型增生组织中呈异常表达,且该基因蛋白与胃癌的临床病理因素相关,提示Survivin在胃癌的发生发展中起重要作用; (2)在胃癌及异型增生组织中,Survivin的表达与p53、 bcl-2基因蛋白存在相关性,提示3种基因蛋白可能在胃癌的发生、发展中起协同作用,联合检测Survivin、p53、 bcl-2的表达,不仅有助于了解胃癌的发病机制, 还可作为癌前或交界性病变发展演变的监测指标。
【关键词】胃癌 Survivin 凋亡抑制免疫组织化学
Abstract Objective To study the expression of survivin and its relationship with the expressions of p53 and bcl-2 in gastric adenocarcinoma and dysplasia. Methods The expressions of surviving, p53 and bcl-2 were measured by streptavidin-peroxidase immunohistochemistry in 50 cases of gastric adenocarcinoma , 32 cases of dysplasia and 20 cases of normal gastric mucosa. Results The positive rates of survivin in gastric cancer, mild, moderate and severe atypical hyperplasia were 56%, 9%, 10% , %, respectively ; The positive rates of survivin in mild and moderate dysplasia were significantly lower than those in severe dysplasia and carcinoma ; No significant difference was observed in survivin expression level between sever