文档介绍：TGF-β1对胃癌细胞和耐药性胃癌细胞生长及p16,cyclin D1蛋白表达的影响
(作者:___________单位: ___________邮编: ___________)
作者:李柱虎,任香善,李美子
[摘要] [目的] 探讨TGF-β1对胃癌细胞株SNU-601/WT和耐药性胃癌细胞株SNU-601/cis2生长p16,cyclin D1蛋白表达的影响. [方法] 将经TGF-β1处理SNU-601/WT,SNU-601/cis2细胞作为实验组,未经处理的作为对照组,利用MTT法检测TGF-β1对两组细胞生存率的影响,并利用免疫细胞化学方法检测各组细胞内p16,cyclin D1蛋白表达情况. [结果] 实验组SNU-601/WT,SNU-601/cis2细胞生存率呈时间依赖性下降,与对照组比较均有显著性差异;SUN-601/WT,SNU-601/cis2实验组细胞内p16蛋白表达呈时间依赖性增强,而cyclin D1蛋白表达则无明显变化. [结论] TGF-β1具有抑制SNU-601/WT胃癌细胞和SNU-601/cis2耐药性胃癌细胞生长作用,其机制可能与增强p16蛋白表达有关,而与cyclin D1蛋白无明显相关性.
[关键词] 胃肿瘤;耐药性;基因,p16
ABSTRACT:OBJECTIVE To investigate the effects of TGF-β1on the cell growth of gastric cancer cell line SNU-601/WT and drug resistant cell line SNU-601/cis2and the expression of p16and cyclin SNU-601/WT and SNU-601/cis2cell lines were divided into the experimental and control effect of TGF-β1on survival rate of these cell lines were measured by MTT protein expression of p16and cyclin D1were detected by immunocytochemical In the experimental group,the growth rate of SNU-601/WT and SNU-601/cis2were decreased along with the time were significantly pared with the control the mean while,the growth rate of drug resistant cell line was lower than that of the gastric cancer cell line -β1was a time-dependent increase incells positive for p16expression in