文档介绍：白藜芦醇体外及小鼠体内抗白血病作用机制
(作者:___________单位: ___________邮编: ___________)
【摘要】目的:探讨白藜芦醇(resveratrol, Res)体内外抗白血病作用的分子机制。

方法:采用HuT78、Jurkat和L1210 3种白血病细胞株,不同浓度Res作用不同时间后,应用甲基噻唑基四唑(methyl thiazolyl tetrazolium, MTT)比色法检测细胞增殖情况,流式细胞仪检测细胞凋亡,蛋白印迹法检测Bcl2和Bax蛋白表达,免疫沉淀法检测信号转导子和转录激活子(signal transducers and activators of transcription,STAT)3及其磷酸化活性(phosphoTyr705STAT3, pSTAT3)。BALB/、25、50 mg/(kg·d) Res组,皮下注射L1210细胞建立白血病模型。逆转录聚合酶链式反应(reverse transcriptionpolymerase chain reaction, RTPCR)检测各组白血病小鼠外周血T淋巴细胞白细胞介素6(interleukin6, IL6)基因表达,流式细胞仪检测IL6蛋白分泌,免疫组织化学法和蛋白印迹法检测肝脏活性p
STAT3蛋白表达。

结果:Res能抑制白血病细胞HuT78、Jurkat、L1210的生长增殖,诱导细胞凋亡,同时明显下调Bcl2/Bax比值、降低活性pSTAT3的蛋白表达;Res还能够剂量依赖地下调白血病小鼠外周血T淋巴细胞IL6 mRNA表达及其胞内分泌,同时降低小鼠肝组织中活性pSTAT3蛋白表达水平。

结论:Res体内外均具有明显的抗白血病效应,其作用机制可能与其对IL6及pSTAT3的影响有关。
【关键词】白藜芦醇; 细胞凋亡; 白血病; 白细胞介素6; STAT3转录因子; 信号转导接头蛋白类; 小鼠; 体外研究
Methods: Three kinds of leukemia cell lines, HuT78, Jurkat and L1210 cells, were used in this study. After different doses of Res treatment, methyl thiazolyl tetrazolium (MTT) colorimetry was used to detect the cell proliferation; apoptosis was detected by flow cytometry; Western blot and immunoprecipitation method were used to detect the Bcl2 and Bax proteins expression and the activity of phosphosignal transducer and activator of transcription 3 (pSTAT3). Besides, a total of 40 BALB/c mice were randomly divided into untreated group and , 25 and 50 mg/(kg
·d) Res groups. Then, leukemiabearing model was established by L1210 cells subcutaneous injection. Interleukin6 (IL6) was detected to determine the secretion function of T lymphocytes of the mice; the IL6 mRNA expression in the liver tissue of mice was also detected by reverse transcriptionpolymerase chain reaction (RTPCR), and the expression of the pSTAT3 protein was measured by Western blot and immunohistochemical method.
Results: The results indicated that resveratrol could inhibit the proliferation of HuT78, Jurkat and L1210 cel