文档介绍：19 病毒对先天性心脏病先天感染的探证及电镜观察
作者:王晓明张国成韩美玉
【关键词】先天性心脏病
关键词: 先天性心脏病;微小病毒B19 ;显微镜检查,电子
摘要:目的证实部分先天性心脏病(CHD)患者心肌组织中微小病毒B19 系先天感染所致,并观察CHD心肌组织超微结构变化. 方法采用ELISA,PCR,原位杂交(ISH)及透射电镜等技术,对42例CHD患者及对照组22例风湿性心瓣膜病患者的外周血、活检心肌组织分别进行B19 -VP2 -Ig M,B19 -DNA的检测,并用透射电镜对心肌细胞的超微结构进行观察. 结果①CHD组中B19 -VP2 -IgM阳性3例(7%),对照组中阳性1例(5%),两组间无显著差异(P>);②CHD组中B19 -DNA阳性8例(19%),与对照组均阴性相比有显著性差异(P<),且与8例心肌B19 -DNA阳性标本配对的血中B19 -VP2 -IgM均阴性;③ISH显示B19 DNA定位于心肌细胞核内,电镜超微结构观察细胞胞质、核内均未见23nm病毒样颗粒,也无细胞器的损害. 结论 B19 病毒对先天性心脏病的感染系先天性感染所致,病毒基因可能整合于宿主细胞核内.
Keywords:congenital heart disease;parvovirus B19 ;mi-croscopy,electron
Abstract:AIM To test if the parvovirus B19 infection in car-diac tissue in congenital heart disease(CHD)is congenital,and to observe the ultrastructural features of cardiac tissue of By using ELISA,B19 -VP2 -IgM was detected in peripheral blood,and using PCR and ISH,B19 -DNA was detected in cardiac tissue from42patients with congenital heart disease and22health controls;ultrastruc-ture was observed by electron microscopy for B19 -DNA posi-tive cardiac tissue of ①B19 -IgM positive rates %%in peripheral blood of CHD and controls, was no significant difference(P>).②B19 -DNA positive rates %and0%in cardiac tissue of CHD and control significant difference was seen(P<),and③B19 -DNA was locate