文档介绍：PRRSV人工单独感染及其与PCV2人工联合感染的病理学比较
【关键词】病理变化
摘要:目的比较猪繁殖与呼吸综合征(PRRS)和断奶仔猪多系统萎缩综合征(PMWS)的病理学变化。方法用PRRSV VR-2332株和猪圆环病毒2型(PCV2)联合感染了2头3周龄仔猪,同时设立了3头健康对照猪。在攻毒前后分别于耳缘静脉采血,用HerdChek IDEXX Kit测其血清抗体。PRRSV和PCV2联合感染猪分别在感染后1周、3周剖杀,PRRSV单独感染猪和健康对照猪分别在感染后1周、2周和3周剖杀。剖杀后采集肺、脾、扁桃体和腹股沟淋巴结,用甲醛溶液固定,组织石蜡切片以观察显微病理变化。结果剖检结果和石蜡切片结果均显示了试验猪在攻毒后发生程度不一的间质性肺炎及相应的显微病变。结论 PRRSV和PCV2联合感染引起的病变比PRRSV单独感染要严重。
关键词:猪繁殖与呼吸综合征病毒;猪圆环病毒;病理变化
Abstract:Objective pare the pathology of PRRSV infection with that of co-infection of PRRSV and Three3-week-old pigs were experimentally infected with PRRSV VR-2332strain,and two pigs with both PRRSV VR-2332and age-matched pigs were kept as uninoculated
as collected before and after inoculation to detect the PRRSV infected pigs and one control pigwere killed and necropsied each time,one,two and three weeks after ,tonsil,spleen and inguinal lymph node were collected for Interstitial pneumonia of different degrees was niticed in all inoculated Coinfection with PCV2would in-crease the severity of PRRSV-induced pneumonia.
Key words:porcine reproductive and respiratory syndrome virus(PRRSV);porcine circovirus(PCV);pathology
猪繁殖与呼吸综合征(PRRS)是猪的重要传染病,于1987年首次在美国发现,随后迅速蔓延至全球各养猪业发达国家。1991年由荷兰学者分离到该病的病原,并命名为Lelystad病毒(PRRSV欧洲型代表株)[1-2] ,1992年美国研究人员也分离到PRRSV(