文档介绍：目录
中文摘要 1
英文摘要 1
综述 2
1 病原 3
2 流行病学 3
3 临床症状及病理变化 4
4 发病机理 5
5 诊断 5
6 防制 5
严格环境消毒 6
尝试人工免疫 6
重视仔猪护理 6
防止免疫抑制 6
论文篇 7
8
实验动物与接种病毒: 8
实验动物 8
病毒制备 8
8
8
8
8
8
9
HE染色 9
2 结果 9
9
9
12
致谢 14
参考文献 14
PCV2人工感染仔猪的病理学观察
动医0401:张伍
指导老师:王小波
摘要:用PCV2毒株接种60日龄长白-太湖二元杂交商品猪,肌注接种21天后,剖检,采集支气管淋巴结、腹股沟浅淋巴结、纵隔淋巴结、肠系膜淋巴结、髂下淋巴结、十二指肠、结肠、肾脏、胰腺、扁桃体、脾、心、肝、大脑、小脑和胸腺置于10%福尔马林固定,制作石蜡切片,经HE染色后观察组织学病理变化。剖检变化轻微,只表现为全身淋巴结轻度肿大,间质性肺炎和肝脏轻度肿大淤血。组织病理学变化表现为淋巴结、脾和扁桃体等免疫器官淋巴滤泡萎缩,淋巴细胞数量减少,有大量巨噬细胞浸润,肺脏呈典型的间质性肺炎,肝脏内可见局灶性坏死及淋巴细胞浸润。
关键词:PCV2;人工感染;仔猪;病理变化
Pathological Changes of Piglets Infected with Porcine Circovirus Type 2
Graduate: Zhang Wu
Advisor: Wang Xiao-bo
Abstract: Two pigs were inoculated intranasally and intramuscular with 2× TCID50 PCV2 virus. necropsies were performed at 21days post inoculation. and tissue samples for histopathologic examination were taken from lungs , heart, lymph nodes (tracheobronchial, mediastinal, mesenteric, subiliac, and superficial inguinal), tonsil, thymus, liver, spleen, small intestine, colon, pancreas, and kidney.
Only mild clinical signs of disease were observed, mild enlargement of lymph nodes was the most obvious lesion, other lesions were non-collapsed lungs, mild hepatic congestion. Varying degrees of lymphocellular depletion, affecting both lymphoid follicles and parafollicular zones, and progressive multifocal to diffuse infiltration of lymph nodes. lung lesions characterized as severely peribronchiolar lymphoplasmacytic and histiocytic bronchointerstitial pneumonia and liver lesions characterized as mild lymphoplasmacytic hepatitis were detected in piglets.
Key words: PCV-2 ; infection; piglets; pathological changes
综述
猪圆环病毒感染( Porcine circovirus infection)是由猪圆环病毒引起的猪的一种新的传染病。其临诊表现多种多样,主要特征为体质下降、消瘦、贫血、黄疸、生长发育不良、腹泻、呼吸困难、母猪繁殖障碍、内脏器官及皮肤的广泛病理变化,特别是肾、脾脏及全身淋巴结高度肿大、出血和坏死。本病还可导致猪群产生严重的免疫抑制,从而容易导致继发或并发