文档介绍：Stathmin基因反义核酸对人成骨肉瘤细胞系的生长抑制作用
作者:范熙明张惠中张明华范清宇
【关键词】成骨肉瘤
关键词: Stathmin基因;反义核酸;成骨肉瘤;细胞系
摘要:目的探讨Stathmin基因的反义核酸(AS-ODN)对人成骨肉瘤细胞系的生长抑制作用,并观察与化疗药物合用后的协同作用. 方法以高表达Stathmin的人成骨肉瘤细胞系SOSP-9607为靶细胞、反义Stathmin(AS-ODN)为阻断剂,通过MTT试验观察AS-ODN对成骨肉瘤细胞的生长抑制作用及与紫杉醇(PTX)的协同作用,流式细胞仪分析细胞增殖周期的影响. 结果 AS-ODN及AS-ODN与PTX联合应用均明显抑制成骨肉瘤细胞的生长(P<,P<).SOSP-9607在AS-ODN作用下,细胞分裂阻滞在分裂期的中期,并诱导细胞发生凋亡. 结论 Stathmin基因的反义核酸(AS-ODN)对成骨肉瘤细胞的生长可能起十分重要的作用,它有望成为成骨肉瘤治疗的新靶点,与抗癌药联合应用有协同作用.
Keywords:Stathmin gene;antisense oligonucleotide(AS-ODN);a;cell line
Abstract:AIM To investigate the inhibition of AS-ODN of Stathmin gene on cultured human a cell lines and to observe the synergistic action when co
mbined with anti-cancer chemical Human a cell lines SOSP-9607,with high expression of stathmin,were used as target cells and AS-ODN for blocking agent,to ob-serve the inhibitory effects of AS-ODN as cultured human a cell lines and the synergistic action -bined with Paclitaxel by using MTT influence of cellproliferation cycle were analyzed with flow The growth of a cells were sup-pressed by AS-ODN(P<)and union of AS-ODN with Paclitaxel(P<).Under action of AS-ODN,the cell di-vision was blocked in metaphase and cell apoptosis was in┐ The AS-ODN of Stathmin gene may play a crucial role in the growth of a cells,it may be the new