文档介绍：CpG ODN增强乙肝疫苗免疫反应的研究
作者:戴帆,董斌,郭晓磊,石碧珠,方丽娟,莫家琳,田素娟,孟民杰
【摘要】目的研究人工合成的CpG ODN对乙肝表面抗原的免疫增强效应,评价CpG ODN的佐剂效应。方法将不同剂量特定的CpG ODN与乙肝表面抗原联合免疫小鼠,用ELISA检测小鼠乙肝表面抗体的滴度。结果 CpG ODN联合乙肝表面抗原免疫小鼠,在短期检测中,高剂量组能较快的达到检测阈,各剂量组间抗体滴度检测值差异有显著性(P<),在长期检测中,各剂量组间抗体滴度检测值差异有显著性(P<),检测值随CpG剂量的增高而增大。结论 CpG ODN增强乙肝表面抗原的免疫反应短期和长期都具有剂量效应。
【关键词】 CpG ODN 乙肝表面抗原免疫佐剂体液免疫
Abstract:Objective To investigate the effects of immunological enhancement of HBsAg with synthetic CpG oligodeoxynucleotides,evaluate the adjuvant effect of CpG ODN. Methods Mice were inated with CpG oligodeoxynucledtides and HBsAg at different doses,and then titers of HBsAb in the blood serums of mice were detected with ELISA. Results CpG ODN and HBsAg immunize the BALb/c mice,the highdose group could be detected quickly in shot term,the levels of antibodies in groups which use different doses of CpG ODN and HBsAg were different in statics(P<),in long term the levels of antibodies in groups which use different dose
s of CpG ODN and HBsAg were different in statics(P<),the levels of antibodies were corresponded with the levels of CpG ODN (P<).Conclusion The immunological effect of HBsAg was enhanced with CpG ODN as adjuvant in short and long terms.
Key words:CpG ODN; HBsAg; immunoadjuvant; humoral immunity
CpG基序是一种具有非特异性免疫刺激能力的DNA序列,为非甲基化的胞嘧啶鸟嘌呤二核苷酸重复序列。CpG ODN (unmethylated CpG motifbased oligodeoxynu