文档介绍：枸杞多糖对实验性肝癌小鼠肿瘤细胞FasL表达的影响及其抗肿瘤作用机制
作者:何彦丽,杜标炎,王慧锋,苏宁,肖霞
【摘要】【目的】观察枸杞多糖(LBP)对实验性肝癌小鼠肿瘤细胞死亡分子Fas配体(FasL)表达的影响,探讨其抗肿瘤作用机制。【方法】选用昆明种小鼠,随机分为模型组,LBP低、高剂量组(剂量分别为0625、1250 g·kg-1·d-1),各组均采用右侧腋下接种H22肝癌腹水型细胞复制荷瘤模型,从第3天开始按设计剂量给药,连续14 d。采用苏木素—伊红(HE)染色观察各组肿瘤细胞密度、细胞核分裂计数及间质淋巴细胞浸润情况,采用免疫组化法观察各组小鼠肿瘤细胞FasL表达情况。【结果】 LBP低、高剂量组均可显著降低肿瘤细胞核分裂计数(P<005);LBP高剂量组可升高肿瘤间质淋巴细胞浸润程度(P<005),HE染色显示在肿瘤边缘组织内见多量淋巴细胞浸润。LBP低、高剂量组均可显著降低小鼠肿瘤细胞FasL阳性表达指数(P<005);免疫组化染色显示模型组FasL表达强阳性,LBP低剂量组部分肝癌细胞内FasL表达阳性,FasL仅在LBP高剂量组少量肝癌细胞中有表达。【结论】 LBP抗实验性肝癌的效应可能与其能抑制FasL表达,减少免疫活性细胞凋亡有关。
【关键词】枸杞多糖/药理学肝肿瘤/中药疗法肿瘤组织/病理学基因表达调控疾病模型,动物小鼠
Abstract: ObjectiveTo observe the influence of Lycium Barbarum haride(LBP) on FasL expression in H22bearing mice and to explore its antitumor mechanism. MethodsKunming mice were randomized into the model group, and low and highdose LBP (in the dose of 0625 and 1250 g·kg-1·d-1 respectively) groups. H22bearing mice models were induced through right subaxillary inoculation of H22ascitic cells. Three days after inoculation, LBP group were given LBP for 14 days. Hematoxylin and eosin(HE) staining method was used to examine the tumor cell density, tumor cell mitotic count, and lymphocyte infiltration in tumor interstitial tissue. FasL expression was observed in the three groups with immunohistochemical method. ResultsTumor cell mitotic count was decreased in the two LBP groups (P&lt;005), the score of lymphocyte infiltration in tumor interstitial tissue was increased,and lymphocyte infiltration was obvious after HE staining in highdose LBP group (P&lt;005). The index of FasL expression positive was decreased in both LBP groups (P&lt;005).FasL expression was strong positive in the model group, and positive in partial liver tumor cells of lowdose LBP group and in less liver tumor cells of highdose LBP group. ConclusionThe antitumor mechanism of LBP is probably related with the inhibition of FasL expression and with the suppression of apoptosis of immune active lympho
cytes.