文档介绍：枸杞提取物的抗氧化作用
作者:杨薛康,海春旭,梁欣,赵海龙,柏桦
【关键词】枸杞;化学发光测定法;抗氧化作用;脂质过氧化作用
Antioxidation of Lycium barbarum extracts
【Abstract】 AIM: To investigate the antioxidative activity of the ethanol and water extracts of Lycium barbarum and their doseeffect relationship. METHODS: The Lycium barbarum extracts were provided by our laboratory. SpragueDawley rats were killed and then the livers were harvested to isolate the microsomes by calcium precipitation. The oxidantscavenging potential of the water and alcohol extracts was measured using the following chemiluminescence systems: CoCl2/EDTAinduced luminol chemiluminescence method and xanthinexanthine oxidaseluminol method. Two types of lipid peroxidation of rat liver microsomes models were used to determine the antioxidation of Lycium barbarum extracts. Then the content of malondialdehyde (MDA) was tested by colorimetry for analyzing the antioxidation of Lycium barbarum extracts in the 2 models. RESULTS
: In the 2 chemiluminescence systems, compared with control group, the inhibition rate of ・OH and O2-・ by different dosages of the 2 extracts significantly increased(P<),and there was a linear doseeffect relationship between Lycium barbarum and luminous intensity; the IC50 of water extract was and , and that of ethanol extract was and . In the VC/Fe2+, CHP model, the content of MDA in those groups with