文档介绍：红藻氨酸诱导大鼠癫痫发作时海马mGluR5mRNA表达变化的动态观察
作者:江文黄远桂王洪典肖华胜高华
【关键词】谷氨酸受体
关键词: 谷氨酸受体;癫痫;海马;红藻氨酸;脑电图
摘要:目的探讨红藻氨酸(KA,10mg・kg-1 )诱导大鼠癫痫发作时海马代谢型谷氨酸受体5亚型(mGluR5)mRNA表达变化的病理特征. 方法采用同位素S35 dATP标记寡核苷酸探针原位杂交法检测大鼠癫痫发作前后海马mGluR5mRNA的表达变化,. 结果 KA注射后大鼠均出现严重边缘性惊厥,,,CA1,CA3,CA4区表达在2h呈现出显著性差异(P<),齿状回区表达在1h即有显著性差异(P<),至72h各区表达均至最低. 结论 mGluR5在癫痫发作后表达下降可能有助于限制神经元网络的异常兴奋性,使细胞发挥自身保护性作用.
Abstract:AIM To investigate temporospatial changes of metabotropic glutamate receptor subtype5(mGluR5)mRNA expression in rat hippocampus during seizures induced by kainic acid(KA).METHODS Using in situ hybridization with S35 dATP-labelled oligodeoxyribonucleotide probes,mGluR5mRNA expression was relative num-ber of hybridization signals was quantified by image analysis electroencephalogram(EEG)and behaviour changes of the rats were simultaneously All rats of KA-injected group exhibited severe limbic con- EEG recordings also showed electrical seizure was widely expressed in rat hip-pocampus with the higher levels in the granular layer of the dentate gyrus and the pyramidal layer of KA in-jection,mGluR5mRNA expression exhibited time-dependent scanning revealed that mGluR5mRNA was mar