文档介绍：灵芝对高糖培养的大鼠肾小球系膜细胞MMP-2和TIMP-2表达的影响
【摘要】目的探讨灵芝对在高糖刺激下大鼠肾小球系膜细胞(MCs)表达基质金属蛋白酶-2(MMP-2)及基质金属蛋白酶-2组织抑制剂(TIMP-2)的影响。方法大鼠MCs在高糖培养下,予不同质量浓度灵芝、苯那普利刺激后,运用免疫组织化学技术和图像分析法,观察MCs表达MMP-2和TIMP-2的变化, RT-PCR 检测MMP-2、TIMP-2 mRNA表达水平。结果不同质量浓度的灵芝对高糖培养的大鼠MCs MMP-2和TIMP-2表达的影响不同。与高糖组相比,随着灵芝浓度逐渐增加,MMP-2的表达逐渐增加,TIMP-2的表达逐渐降低。结论灵芝通过改变高糖培养的大鼠MCs MMP-2和TIMP-2的表达量,影响糖尿病肾病(DN)的进程。
【关键词】灵芝;基质金属蛋白酶-2; 基质金属蛋白酶-2组织抑制剂;系膜细胞;大鼠;糖尿病肾病
Abstract:Objective To investigate the effect of Ganoderma lucidum on the expressions of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) by rat glomerular mesangial cells (MCs) cultured under high-glucose The culture of rat MCs was continued under high glucose level for 48 h before the experiment. Following the addition of Ganoderma lucidum of varied concentrations or benazepril, the changes in the expression of MMP-2 and TIMP-2 in the MCs and the mRNA levels of them were examined by immunohistochemical method, image analysis and RT-PCR The levels of MMP-2 and TIMP-2 were found varying with the concentration of Ganoderma lucidum, . MMP-2 increased and TIMP-2 decreased with the increase of Ganoderma lucidum Ganoderma lucidum may modulate the process of diabetic nephropathy by changing the expression of MMP-2 and TIMP - 2.
Key words: Ganoderma lucidum; matrix metalloprot