文档介绍：睫状神经营养因子和雪旺细胞神经营养活性物质对大鼠视神经损伤后轴突数的影响
【关键词】眼损伤
关键词: 眼损伤;视神经;睫状神经营养因子;雪旺细胞神经营养活性物质;大鼠
摘要:目的观察睫状神经生长因子(ciliary neurotrophic TF)和雪旺细胞神经营养活性物质(Schwann cells neurotrophic agent,SCNA)对视神经损伤后轴突的形态和纤维数目变化的作用. 方法采用镊夹法制作大鼠视神经损伤模型,TF(50ng)A(10μL),在损伤后1,2,3和4wk时观察夹伤视神经的形态,及进行图像分析和轴突纤维计数. 结果损伤后视神经轴突减少,A组的轴突数密度在1wk时分别为正常值(±・μm-2 ),,实验组与对照组比较差异显著(P<).但在夹伤2wk后3个组间、各时间点间数据差异均不显著(P>). A一次玻璃体给药能在1wk内减缓视神经损伤后轴突数目的减少.
Keywords:eye injury;optic nerve;ciliary neurotrophic fac-tor;Schwann cells neurotrophic agent;rat
Abstract:AIM To study the effects of ciliary neurotrophic TF)and Schwann cell neurotrophic agent(SCNA)on changes of configuration and the number of nerve fibers in a rat’s model of the crushed optic The optic nerve injury model of rats was induced by crushed at the optic nerve1mm an intravitreal injec-tion TF(50ng)or SCNA(10μL)was cross-section morphology changes were observed1,2,3and4weeks after injury,and the number of nerve fibers was de-termined by using puterized image analysis system at the time The axon number of crushed op-tic nerve decreased but the matrix axon num-ber density of TF group and the SCNA group the normal level(±)one week after injury,while the control group’s (P<).But there was no difference in three groups2,3and4weeks after An intravitreal injection TF or SCNA can reduce the loss of optic nerve fibers in crushed optic nerve,so far only noted within one week after injury.
0 引言
19世纪末至20世纪初,科学家们发现低等脊椎动物如鱼类和两栖类的中枢和外周神经系统(PNS),只有外周神经系统损伤后可以再生,S)则不能[1] .直到80年代初So等[2] 发现体外中枢神经可以再生,体内神经细胞的轴突不能再生的原因可能为环境因素和抑制因素所致,从此该领域的研究取得一些突破性成果,特别是近十年来的研究工作使人确信,,,往往引起视力丧失[3] .由于视神经属于中枢神经系统,损伤后缺乏神经修复和再生所需的微环境和物质,因此视神经损伤后的治疗和功能恢复是临床上的一个难题[4] .TF)能够促进多种神经细胞的存活,在神经系统发育、分化和损伤修复中具有非常重要的作用[