文档介绍：胃癌及其癌前病变中ras,c┐erbB┐2,p53癌基因产物的表达
作者:惠延平黄高升王文亮王映梅朱晓慧马福成
【关键词】胃肿瘤
关键词: 胃肿瘤;癌前状态;基因表达;免疫组织化学
摘要:目的探讨胃癌及其癌前病变中ras,c-erbB-2,p53癌基因蛋白产物的表达分布特点及其在胃癌发生发展中的作用. 方法应用免疫组化ABC方法,对58例胃癌、31例胃粘膜上皮异型增生和11例正常胃粘膜组织中,鼠抗人mAb ras基因p21蛋白、兔抗人c-erbB-2原癌基因产物多克隆抗体p185,鼠抗人mAb p53蛋白进行了检测. 结果 p21在正常胃粘膜和轻、中、%,%,%,%%;其表达率在重度异型增生和胃癌中显著高于正常胃粘膜和轻、中度异型增生(P<);p185在正常胃粘膜和轻、中度异型增生中无表达,%,%,各组间无显著性差异(P>);p53仅在癌中有表达,%,明显高于非癌组织(P<).胃癌中两种以上癌基因蛋白同时表达率明显高于非癌组织(P<). 结论 ras基因可能作用于胃癌发生的早期阶段,c-erbB-2,.
Keywords:stomach neoplasm;procancerous condition;gene expression;immunohistochemistry
Abstract:AIM To investigate the expression and distribu-tion features of p21,p53and p185oncoproteins in gastric cancer and its precancerous Immuno-histochemical method was used to measure tissues of58gas-tric cancer and31gastric mucosa Pos-itive rate of p21in mild,moderate,severe dysplasia and can-cer of stomach was3/10(%),6/13(%),6/8(%)and42/58(%)respectively;the positive rate of p185in severe dysplasia and cancer of stomach was2/8(%)and11/58(%)resprectively;p53expression was only in cancer17/58(%).The positive percentage of ras p21in severe dysplasia was significantly higher than that of normal mucosa or mild dysplasia(P<