文档介绍：银杏叶提取物对6-OHDA诱导PC12细胞氧化应激的影响
【摘要】目的探讨银杏叶提取物(Ginkgo biloba extract, GBE)对6-羟基多巴胺(6-hydroxydopamine, 6-OHDA)诱导的PC12 细胞氧化应激的保护作用及其机制。方法经不同剂量GBE预处理体外培养的PC12细胞后,加入6-OHDA诱导多巴胺能神经元损伤模型。用MTT法检测PC12细胞活力;分光光度法测定细胞过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、总超氧化物歧化酶(T-SOD)的活性和总抗氧化能力(T-AOC)的水平;硫代巴比妥法测定细胞丙二醛(MDA)含量。结果 100 μmol/L的6-OHDA处理PC12细胞24 h,细胞活力较正常对照组明显降低(P<),CAT、GSH-Px、T-SOD的活性和T-AOC的水平明显降低(P<),MDA含量明显升高(P<);与模型组比较,不同剂量GBE预处理组的PC12细胞活力逐渐升高,CAT、GSH-Px、T-SOD的活性和T-AOC的水平明显升高(P<),MDA含量明显减少(P<)。结论 GBE对6-OHDA诱导的PC12 细胞氧化应激具有抑制作用,提高CAT、 GSH-Px、T-SOD的活性和T-AOC的水平可能是其抗氧化作用的机制之一。
【关键词】银杏叶提取物;6-羟基多巴胺;PC12细胞;氧化应激
Abstract: Objective To explore the protective effects and the causal mechanism of Ginkgo biloba extract (GBE) on the oxidative stress induced by 6-hydroxydopamine (6-OHDA) in PC12 cells. Methods Dopaminergic neuronal injury model was induced by addition of 6-OHDA into PC12 cells cultured in vitro and pretreated with different doses of GBE. Cell viability, the levels of four antioxidative indexes [including total superoxid dismutase (T-SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC)] and the content of malondialdehyde (MDA) were assayed by MTT, visible spectrotometry and the method of thio
barbituric acid reaction, respectively. Results The cell viability of PC12 cells treated with 100 μmol/L 6-OHDA for 24 h was lowe