文档介绍：紫外线照射对储存血中淋巴细胞功能的影响
作者:王宝燕,张建耕,刘淑会,朱红梅,王保平,魏省禄
【关键词】淋巴细胞
【Abstract】 AIM: To explore the effects of ultraviolet irradiation on the function of lymphocytes in banked blood. METHODS: Changes of lymphocyte subgroups, lymphocyte activity, lymphocyte transformation, interleukin2 and its receptor were tested before and after ultraviolet irradiation, and after storage for 72 h separately. RESULTS: Ultraviolet irradiation inhibited the lymphocyte activity. pared with that of the control group, the content of interleukin2 decreased significantly with prolonged storage time(P<). Ultraviolet irradiation markedly inhibited the lymphocytes transformation(P<). CD3+, CD4+ and CD8+ lymphocyte subgroups in banked blood decreased pared with those of prestorage in the two groups,but there was no significant difference between the two groups. CONCLUSION: Ultraviolet irradiation can inhibit lymphocytes activity and lymphocytes transformation and reduce the accumulation of cytokine in banked blood.
【Keywords】 ultraviolet rays; blood preservation; lymphocytes
【摘要】目的: 研究紫外线照射对储存血中淋巴细胞功能的影响. 方法: 在血标本经紫外线照射前后及储存72 h后测定淋巴细胞亚群及活性、细胞因子. 结果: 紫外线照射储存血可抑制淋巴细胞的活性. 照射后随储存时间的延长,血液中IL2水平与对照组相比明显降低(P<);经紫外线照射后淋巴细胞转化率较对照组明显降低(P<);对照组与照射组血液在放置72 h后CD3+,CD4+,CD8+细胞亚群均有明显下降,但无显著差异. 结论: 紫外线照射储存血可抑制淋巴细胞的活性,对淋巴细胞的增殖能力有明显抑制作用;可减少细胞因子的产生和积累.
【关键词】紫外线;血液保存;淋巴细胞
0引言
输入含有免疫活性淋巴