文档介绍：IL-6对照射小鼠脾淋巴细胞亚群活性的影响
作者:王红兵,王绪,刘永彪,郝兴芝
【关键词】对照
摘要:目的研究白细胞介素6(IL-6)对照射小鼠脾淋巴细胞亚群自然杀伤活性的影响。方法 、对照组和照射+IL-6组,采用Panning法从小鼠脾淋巴细胞中分离L 3 T 4 、Lyt 2 和B细胞,用 3 H-TdR释放实验检测脾淋巴细胞亚群自然杀伤活性。结果照射+IL-6组与对照组比较, 3 H-TdR释放量有显著性差异(P<)。结论 IL-6具有促进照射小鼠脾淋巴细胞亚群活性作用。
关键词:小鼠;分次照射;淋巴细胞;白细胞介素6;自然杀伤活性;K562;S180
Abstract:Objective To study the effects of IL-6on the natural killing activity of spleen lymphocyte subsets in irradiat-ed The spleen lymphocytes in irradiated mice were separated into L3T4,Lyt2and B cells by a panning meth- natural killing activity of spleen lymphocytes was determined by means of 3 H-TdR release Significant differences in 3 H-TdR release were f
ound between the IL-6group and the control group(P<).Conclusion IL-6is able to stimulate the killing activity of spleen lymphocyte subsets in irradiated mice.
Key words:mice;fractional irradiation;lymphocyte;IL-6;natural killing activity;K562;S180
白细胞介素6(IL-6)是一种生物活性非常广泛的细胞因子[1] ,可作用于淋巴细胞,诱导其增殖、分化和生长,还可以通过增强细胞间识别分子的表达,诱发肿瘤细胞对效应细胞的杀伤敏感性增强。我们已证实IL-6对脾淋巴细胞亚群有辐射保护作用[2] ,IL-6是否同时具备促进脾淋巴细胞亚群免疫活性作用?我们就此进行了如下实验。
1 材料和方法
实验动物和肿瘤细胞株 ,体重(20±2)g,由中科院上海实验动物中心提供。K562细胞、S180细胞由中科院上海细胞所提供。
方法
动物分组及处理实验小鼠随机分为3组(n=6)。①照射组:只照射。②照射+IL-6组:于放射后30min尾静脉注射小鼠rIL-6(法国产,苏州大学孔向容博士惠赠)400μg・kg -1 ・d -1 。③对照组:于放射后30min尾静脉注射白蛋