文档介绍：何首乌生品与炮制品薄层层析鉴别方法研究
作者:刘振丽,宋志前,巢志茂,王淳,吕署一,李林福
【摘要】目的研究采用薄层层析(TLC)鉴别方法区别何首乌生品与炮制品的可行性。方法研究样品为来自全国不同地区的3个批次的何首乌样品与10个批次的制何首乌样品,以及道地产地何首乌生品通过不同炮制工艺、不同炮制时间制备的炮制品。TLC鉴别条件:硅胶G薄层板,5-羟甲基糠醛为对照品,以石油醚(60~90 ℃)-乙酸乙酯(1∶1)为展开剂,喷以15%α-萘酚乙醇溶液,在90 ℃加热至斑点显色清晰,日光下检视。结果供试品色谱中,在与对照品色谱相应的位置上,制何首乌显相同颜色的斑点,何首乌没有显示相同颜色的斑点。结论建立的TLC鉴别方法可以区分何首乌生品与炮制品。
【关键词】何首乌;薄层层析;5-羟甲基糠醛
Abstract:Objective To study the method for distinguishing the crude and processed Radix of Polygonum multiflorum Thunb. by TLC. Methods Some of the samples were collected from different cities in China, some of them were prepared following the pharmacopoeia standard or local standard. TLC conditions:5-hydroxymethyl-furfural (5-HMF) was used as a standard matter with the developing solvent of petroleum ether (60~90 ℃)-ethyl acetate (1∶1) on a silica G thin layer plate, sprayed with 15% α-naphthol in ethanol solvent, heated and detected in daylight. Results TLC chromatogram showed that crude and processed product of Polygonum mu
ltiflorum Thunb. were different. Conclusion TLC method for distinguishing the crude and processed Polygonum multiflorum Thunb. was established.
Key words:Radix of Polygonum multiflorum Thunb.;TLC;5-hydroxymethyl-furfural
何首乌为蓼科植物何首乌Polygonum multiflorum ,经炮制后为制何首乌。2005年版《中华人民共和国药典》(一部) [1]对何首乌生品与炮制品采用了相同的薄层层析(TLC)鉴别方法,鉴别的对象为何首乌对照药材,也没有涉及具体的化学成分,故而采用TLC鉴别方法无法区别何首乌生品与炮制品。前期研究发现,何首乌炮制后HP