人参皂甙对应激大鼠肝脏热休克蛋白70表达的影响.doc

文档介绍：人参皂甙对应激大鼠肝脏热休克蛋白70表达的影响
作者:李宁金法吴可人徐涛裘华森
【摘要】目的研究人参皂甙对应激时肝脏热休克蛋白(HSP)70表达的影响,探讨其抗应激的作用机制。方法 54只SD大鼠分为三组:干预组和模型组各24只,分别以人参皂甙溶液或生理盐水灌胃;对照组6只,予正常饮食。采用盲肠结扎穿孔法(cecum ligation and perforation,CLP)制作应激模型。分别于造模后6、12、24及72h检测大鼠肝细胞HSP 70的表达及血清IL-6水平的变化。结果除12h外,干预组肝细胞HSP70的表达均>对照组(P<)。干预组72h肝细胞HSP70表达>模型组(P<)。干预组血清IL-6水平整体呈下降趋势,除6h外,各时点明显<模型组(P<)。结论人参皂甙可提高应激时大鼠肝细胞HSP70的表达,并可抑制IL-6的产生,减轻炎症反应,保护应激时的机体。
【关键词】人参皂甙应激热休克蛋白70 白细胞介素6
【Abstract】Objective To study the effect and mechanism of panaxoside on the expression of liver heat-shock protein-70 in condition of stress. Methods 54 SD rats were divided into panaxoside treating group(n=24),which were fed with % panaxoside liquor at 50mg/kg via gastri
c tube , stress model group (n=24)and normal control group(n=6). Cecum ligature and perforation (CLP) method were used to establish the model. The expression of HSP-70 and serum concentration of IL-6 were evaluated at 6h, 12h, 24h and 72h after operation. Results In panaxoside treating group, the expression of HSP-70 was higher(p<) than normal group at every time point except 12h, and in stress model group it is higher(p<) than in normal group at 72h after operation. IL-6 level were decreased after CLP in panaxoside group, and it was lower in panaxoside group than in stress model group 12h、72h after CLP (p<). Conclusions Panaxoside can promote the expressio