文档介绍：幽门螺杆菌粘附素基因alpA的克隆与高效表达
作者:白杨张亚历王继德杨云生张兆山周殿元
【关键词】,螺杆菌
关键词: 螺杆菌,幽门;粘附素alpA;克隆,分子;基因表达
摘要:目的克隆并表达幽门螺杆菌粘附素AlpA基因. 方法提取幽门螺杆菌染色体基因,用PCR方法扩增alpA基因,将其克隆至表达载体pET-22b(+),转化大肠杆菌BL21(DE3),IPTG诱导表达. ,并在大肠杆菌中实现了该基因的高效表达,在37℃诱导表达3h后,%.表达以可溶性蛋白和包涵体两种形式存在,其中主要是包涵体的形式,%. 结论幽门螺杆菌alpA基因的克隆与表达为Hp疫苗的研制打下了基础.

Keywords:helicobacter pylori;adhesion alpA;cloning mole-cular;gene expression

Abstract:AIM To isolate and expresse alpA gene from He-licobacter The alpA gene was amplified from chromosomal by PCR and inserted into the prokaryotie expression vector pET-22b(+).The binant plasmid was transformed into the BL21(DE3)
li alpA gene was essfully strains expressed AlpA were obtained,the expression protein amounted %of the total bacterial protein after induced with IPTG for3h at37℃.CONCLU┐SION Cloning and high-expression of alpA gene lay the foundation for the development of the ine.

0 引言

幽门螺杆菌(Helicobacter pylori,Hp)已被确认是慢性胃病(包括胃炎和消化性溃疡)的主要病原因子,同时与胃腺癌、胃粘膜相关淋巴组织淋巴瘤的发生亦密切相关[1-6] .目前根除Hp的主要手段是抗菌疗法,,在Hp疫苗的研制中,获得了不同程度的保护作用,,使之在胃蠕动时随食物一起被排空,[7] ,,为构建Hp基因工程疫苗打下了基础.

1 材料和方法

材料菌株BL21(DE3)、质粒pET-22b(+)