文档介绍：柯萨奇B3 病毒抗原决定簇的多态性和致病性
x 作者:朱德生李华向润娥杨信槐王鸣英
【关键词】柯萨奇病毒B组
关键词: 柯萨奇病毒B组;抗体,单克隆;表位
摘要:目的分析由各脏器分离出的柯萨奇B3 病毒(CVB3 )抗原决定簇的异同,并探讨其与致病性的关系. 方法利用自制的26种柯萨奇B3 病毒mAb(CVB3 -McAb)与心脏、肝脏、肾脏及肺脏分离出的CVB3 株进行免疫荧光分析,观察各分离株抗原决定簇的变化,并将各分离株再次注入动物体内,观察心、肝、肾及肺的病理改变. 结果 CVB3 在不同器官的细胞中繁殖后,其抗原决定簇发生改变,CVB3 -McAb2A4 ,2B2 ,2D3 ,2D4 与肝、肺、心、肾分离株均发生反应;26种CVB3 -McAb中有4种不与心脏分离株反应;有15种不与肝脏分离株反应;有21种不与肺脏分离株反应;. 结论 CVB3 各分离株抗原决定簇有差异;病毒抗原决定簇的不同是影响其致病性的重要因素之一.
Keywords:coxackieviruses B;antibod monoclonal;epitopes
Abstract:AIM To analyze antigenic determinant of Cox-sackie virus B3 (CVB3 )separated from ans and to explore its relationship with Im-munofluorence analysis of CVB3 strains separated from heart,liver,kidney and lung with26strains of our self-making Coxsackie B3 monoclonal antibody(CVB3 -McAb)was per-formed,and the alteration of antigenic determinants of the isolates was injected the isolates into animal body again and observed the pathologic alteration of heart,liver,kidney and After the virus repro-duced in ans,Coxsackie B3viral antigenic deter-minant changed,and CVB3 -McAb2A4 ,2B2 ,2D3 ,2D4 all re-acted with the isolates of heart,liver,kidney and did not react with isolates of heart,and15not with isolates of liver,21not with isolates of lung and11not with isolates of pathogenic to