文档介绍：端粒酶RNA特异性核酶抑制卵巢癌细胞端粒酶的活性
作者:王红英辛晓燕马越云苏明权
【关键词】卵巢肿瘤
关键词: 卵巢肿瘤;端粒,末端转移酶;核糖核酸酶类;转染
摘要:目的研究端粒酶RNA特异性核酶对卵巢癌细胞活性的抑制作用. 方法用脂质体法将构建好的核酶逆转录病毒载体转染至卵巢癌HO-8910细胞,以MTT比色法检测细胞的生长,应用流式细胞仪分析细胞周期细胞,观察细胞的增殖情况,同时扩增端粒重复序列,结合杂交分析,检测细胞的端粒酶活性. 结果 MTT检测发现转染核酶后的细胞生长的潜伏期延长(48→72h),对数生长期减缓(4→5d),流式细胞仪结果显示其G1期比例明显增高(%→%,P<),S期细胞比例明显降低(%→%,P<),细胞增殖下降,细胞的端粒酶活性亦降低. 结论端粒酶RNA特异性核酶能抑制卵巢癌细胞端粒酶活性,抑制细胞增殖.
Keywords:ovarian neoplasms;telomere,terminal trans-ferase;ribonucleases;transfection
Abstract:AIM To explore the effects of hammerhead ri-bozyme against human telomerase RNA in ovarian carcinoma We transfected the ribozyme directed a-gainst the ponent of human telomerase into HO-8910,human ovarian carcinoma we observed the propagation of the cells with MTT,analyzed cell cycle phases under FCM and detected their telomerase activity by TRAP-Hyb After transfection,we found that the latent period of the transfected cells prolonged(48→72h)and the logarithm growth period also lengthened(4→5d).FCM showed that cells in G1phase increased obviously(%→%,P<)and decreased obviously in S phase(%→%,P<).The propagation of the cells was inhibited and the telomerase activity was Telomerase ribozyme inhibites the telom-erase activity in ovarian carcinoma cells.
0 引言
核酶(ribozyme,RZ)通过合理的设计