文档介绍：肺气虚大鼠肺、肾组织AQP1表达及血浆ET水平变化
作者:王哲,太史春,赵金茹,李淑玲,王德山
【摘要】目的观察肺气虚模型大鼠肺、肾组织水通道蛋白1(AQP1)表达及血浆中内皮素(ET)水平的变化,为中医学肺肾相关理论提供实验依据。方法 20只大鼠随机分为模型组和对照组,应用放射免疫方法测定血中ET含量;应用Western blot方法测定AQP1蛋白含量的变化。结果肺气虚模型组肾组织AQP1表达高于对照组,而肺组织AQP1表达低于对照组;模型组血浆ET明显升高,与对照组比较,差异有显著性(P<)。结论肺气虚模型大鼠肾组织AQP1表达增强,而肺组织AQP1表达降低,ET可能促进肾组织AQP1表达、抑制肺组织AQP1表达。
【关键词】肺气虚;水通道蛋白1;内皮素;动物模型
Abstract:Objective To observe the changes of lung and kidney aquaporin-1 expression in Lung-Qi deficiency model rats and to investigate pare the effects of endothlin (ET) on the expression of lung and kidney aquaporin-1. It provide expremental basis to the theory of mutual relationship of lung and kidney. Method Twenty healthy rats were divided into the model group and the control group. Sample blood was taken and ET was measured by radioimmunoassay. The method of Western blot was used to observe the expression of aquaporin-1 in lung and kidney. Result The expression of aquaporin-1 in kidney was higher in model group than in the control group, while the expression of aquaporin-1 in lung was on the contrary. Compared with the control group, ET in the plasma was higher in the model group (P<). Conclusion The expression of aquaporin-1 in kidney, was higher in Lung-Qi deficiency model rats and ET promotes the expression of aquaporin-1 in kidney, but on the contrary in lung.
Key words:Lung-Qi deficiency;aquaporin-1;endothlin;