文档介绍：湖北地黄多糖超声波提取工艺条件及含量测定研究
作者:田春莲, 廖博儒,陈建华, 姚玉红, 曾凤
【关键词】超声波提取;,,多糖;,,湖北地黄
摘要:目的建立超声波提取湖北地黄多糖的最佳工艺和测定湖北地黄各部分中多糖的含量。方法对超声波提取湖北地黄多糖的条件进行了研究并用蒽酮-硫酸法测定了湖北地黄各部分的多糖含量。结果由正交实验得出超声波提取的最佳条件为:湖北地黄与水的质量比为1∶40,提取温度为40℃,提取时间为30 min。采用蒽酮-硫酸法于624 nm比色,得湖北地黄中各部分多糖含量为:根、茎、%,%,%。结论湖北地黄的多糖依据不同提取方法、不同部位含量有较大差异。
关键词:超声波提取; 多糖; 湖北地黄
Extraction Technology by Ultrasonic Wave and Content Determination of the haride from Rehmannia glutinosa Libosch. in Hubei Province
AbstractObjectiveTo establish the optimum process of the harides extraction in seeds of Rehmannia glutinosa Libosch. in Hubei province by ultrasonic wave technology and content determination of extraction conditions with ultrasonic wave were studied and the contents of harides were determined by the anthrone-H2SO4 colorimetric optimum process of the technology was abtained through orthogonal test:the ratio of[S]/[L] was 1∶40, the extraction temperature was at 40℃ and the extraction time was 30 min. The contents of the harides were determined by anthrone-H2SO4 colorimetric method (λ=624 nm), and the contents from root, stem and leaf were %, %, % content of the harides in Rehmannia glutinosa Hubei province were different with different extraction mothed and in different part.
Key words:Extraction by ultrasonic wave; haride; Rehmannia glutinosa Libosch. in hubei province
地黄是玄参科植物地黄Rehnannia glutinosch [1]。为常用中药,始载于《神农本草经》列为上品。鲜地黄性寒、味甘苦,能清热生津,凉血止血、养阴。熟地黄性微温、味甘,能滋阴补血、益津填髓,用于肝肾阴虚,月经不调等症,还具有抗肝瘤、抗病毒、增强免疫功能的效果,其中地黄多糖是一种有效的免疫调节剂,具有抗肝瘤、抗病毒、抗衰老等功效[2]。目前国内外主要对于地黄的化学成分、地黄的主要有效成分的药理作用进行研究[3],对怀地黄的多糖提取也有过研究[
4],但对于湖北地黄的多糖提取特别是超声波提取还未见报道,因此本文对湖北地黄多糖的超声波提取进行研究,为深度开发湖北地黄这一资源提供依据。
1 器材与方法
器材
材料湖北地黄于200506采自张家界市永定区后坪镇,洗净,于60℃烘干,粉碎(过40目筛),贮藏备用。
仪器UV160紫外-可见分光光度计(日本岛津)、B8200-J4超声波(日本岛津)、旋转蒸发仪等。
试剂石油醚(60~90℃)、95%乙醇、乙醚、丙酮、蒽酮、硫酸、葡萄糖等均为分析纯。
方法
标准曲线的制备[3] 0 g葡萄糖(105℃烘干至恒重)置