文档介绍：肝豆状核变性T淋巴细胞亚群及NK细胞的变化
作者:汪瀚杨文明鲍远程陈怀珍童建兵
【摘要】目的研究肝豆状核变性(Wilson病,WD)患者的T淋巴细胞亚群及NK细胞亚群变化,并探讨其临床意义。方法应用流式细胞仪直接免疫荧光染色法测定49例WD患者外周血T淋巴细胞亚群及NK细胞亚群,并与健康人组进行对照,同时对不同ChildPugh分级患者的T细胞亚群及NK细胞进行比较分析。结果 WD组CD3+、CD4+、CD8+、CD4+/CD8+较对照组水平无明显变化,NK水平较对照组降低(P<);病情重者CD3+、CD4+、CD4+/CD8+水平仍无差异,随病情加重NK水平显著降低(P<)。结论 WD存在NK细胞免疫失调,而T淋巴细胞免疫功能正常,病情重的患者NK细胞免疫功能更低。
【关键词】 Wilson病;T淋巴细胞亚群;NK细胞亚群;流式细胞仪
【Abstract】 Objective To study the changes and its clinical significance of T lymphocyte subsets and natural kill cells in Wilson’s disease T lymphocyte subsets and natural kill cells were measured in 49 patients by the FCM direct immunofluorescent staining technique pared with normal contro
ls. Meanwhile patients were pared with each other in different ChildPugh The levels of CD3+,CD4+,CD8+,CD4+/CD8+ in WD were normal, the levels of natural kill cells pared with healthy controls(P<). pared with Child-Pugh rating A, the levels of NK were declined more significantly in severe patients who were ChildPugh rating B and C(P<).The difference of CD3+,CD4+,CD4+/CD8+ levels was not T lymphocyte immune function is not difference and immune disturbance of NK are observed in WD patients. The levels of NK are even significantly lower in severe patients.
【Key words】 Wilson’s disease;T lymphocyte;Natural kill cell;FCM
肝豆状核变