文档介绍：河北大学
硕士学位论文
三聚氰胺类物质及某些抗生素的高效液相色谱检测方法研究
姓名:秦晓兰
申请学位级别:硕士
专业:分析化学
指导教师:孙汉文
2011-06
摘要
摘要
环境水体和人体液中三聚氰胺类物质和抗生素的检测对于人的健康具有重要意义。
本文旨在建立有效分离和灵敏检测环境水体中的三聚氰酸、三聚氰胺、环丙氨嗪和尿液
中左氧氟沙星、头孢唑啉钠和头孢米诺钠的反相高效液相色谱新方法。全文分为如下
四个部分。
第一章:简述反相离子对液相色谱分析原理,并简要介绍近年来三聚氰胺及其相关
物质,头孢和氟喹诺酮类抗生素的相关分离检测技术。
第二章:提出了一种新的有效分离和同时检测环境水样中三聚氰酸,三聚氰胺和环
丙氨嗪的反相高效液相色谱法。色谱分离采用了KROMASIL® C18 柱(250 × mm
., 5 μm) 和梯度洗脱程序。所提出的方法具有灵敏度高、精密度好等优点,能够用于
检测环境水样中浓度低至 μg/mL的三聚氰酸和环丙氨嗪,以及浓度低至 μg/mL
的三聚氰胺。
第三章:左氧氟沙星、头孢唑啉钠和头孢米诺钠可口服或静脉注射,并且主要随尿
液排出,其配伍用药可提高药效。提出了一种应用离子对反相高效液相色谱-二极管阵
列检测器,同时测定人体尿样中这两类抗生素的新方法。本方法是有效准确的,其灵
敏度和线性可满足临床浓度水平的检测要求。
第四章:结论与展望。

关键词反相高效液相色谱三聚氰胺头孢抗生素左氧氟沙星
I
Abstract
Abstract
The analysis of melamine-related substances and antibiotics in the environmental waters
and body fluids is of importance for human health. The aim of this paper is to establish the
new reverse phase high performance liquid chromatographic(RP-HPLC)methods for the
effective separation and sensitive determination of melamine, cyanuric acid and cyromazine
in the environmental waters, and levofxaxin, cefazolin and cefminox in human urine. It
includes third chapters as follows:
Chapter 1: The basic principle of reversed-phase ion pair chromatography. The
technolgies concerned with the seperation and determination of melamine-related substances
and some antibiotics in recent years are introduced.
Chapter 2: A new method for effective separation and determination of cyanuric acid,
melamine and cyromazine in the environmental waters by reversed phase high performance
liquid chromatography (RP-HPLC) with diode array detector is developed. A KROMASIL®
C18 column (250 × mm ., 5 μm) was used for RP-HPLC using a gradient elution
procedure. The proposed method has the advantages of high sensitivity and precision, and can
permit the detection of cyanuric acid and cyromazine at levels as low as μg/mL and
melamine at levels as low as μg/mL in