文档介绍:薄层层析分离方法Thin-(moving)!(bottom)-:Silica(SiO2)StationaryPhase:AluminaAcidic:-Al-OHNeutral:-Al-OH+-Al-O-Basic:-Al-O-Thin-LayerChromatography:ponentMixtureMorepolar!Lesspolar!ponentAoriginIncreasingDevelopmentTimeBesuretoremovetheTLCplatewhenitappearsthatthesolventfrontisn’tmoving!Reason::Ifyoudon’tremovetheplateallofthespotswillappearnearthetopoftheplate!!!!!Thisisn’taprettysightandmakesitdifficulttogetgoodRfvalues!Importanthint!,thespotswon’tshowunlesstheyarevisualized!:-LayerChromatography:ponentA=ponentB=dBdSTheRfvalueisadecimalfraction,generallyonlyreportedtotwodecimalplaces