结核分枝杆菌Rel酶相互作用蛋白的筛选.doc

文档介绍：
结核分枝杆菌 Rel 酶相互作用蛋白的筛选#
张华*
(华中农业大学生命科学技术学院,武汉,430070)
5
10
摘要:目的:从结核分枝杆菌全基因组序列中筛选能与结核分枝杆菌 RelMTB 有相互作用的
蛋白,发掘新的 RelMTB 蛋白的活性调控方式;方法:主要采用分子克隆技术克隆了
pBT-relMTB 质粒,并利用细菌双杂交技术筛选与结核分枝杆菌 RelMTB 有相互作用的蛋白;
结果:测序分析表明 relMTB 基因被正确的克隆到 pBT 载体上,经过细菌双杂交筛选发现
47 个能与结核分枝杆菌 RelMTB 有相互作用的蛋白;结论:筛选得到的结核分枝杆菌
RelMTB 的相互作用蛋白提示 RelMTB 可能具有新的活性调控方式,即通过蛋白质-蛋白质
相互作用的方式进行调控。
关键词:微生物;结核分枝杆菌;Rel;鸟苷五(四)磷酸
中图分类号:Q93
15
Screening of proteins interacting with Mycobacterium
tuberculosis Rel
Zhang Hua
(College of life science and technology,Huazhong Agricultural University,Wuhan,430070)
20
25
Abstract: In this paper, the aims were to screen the proteins interacting with Mycobacterium
tuberculosis RelMTB from M. tuberculosis whole genome, and explore its new activity regulation
mechanism. Methods: Molecular cloning techniques were used to clone plasmid pBT-relMTB,
and Bacterial Two-Hybrid system was applied to screen the interaction proteins of RelMTB.
Results: relMTB gene was correctly cloned to vector pBT from sequencing analysis, and 47
proteins were found to interact with M. tuberculosis RelMTB through Bacterial Two-Hybrid
technique. Conclusion: Interaction proteins screened from M. tuberculosis whole genome suggest
that the activity of RelMTB must be a new regulation mechanism, that is protein-protein
interaction.
Key words: Microbiology; Mycobacterium tuberculosis; Rel; (p)p