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玉米大斑病应答相关miRNA的识别及分析.doc

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玉米大斑病应答相关miRNA的识别及分析.doc

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文档介绍:
Analysis of maize miRNA regulated by Exserohilum
turcicum#
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Fangli Wu1, Weibo Jin2**
(1. College of Plant Protection, Northwest A&F University, Yangling, Shannxi, 712100, China.;
2. College of Life Sciences, Northwest A&F University, Yangling, Shannxi, 712100, China.)
Abstract: Northern leaf blight, caused by the fungus Exserohilum turcicum (Pass.), is a major disease
of maize (Zea mays L.). MicroRNAs (miRNAs) have been recently reported as gene expression
regulators related to several stress responses; however, evidence of the role of miRNAs in plant
response to biotic stresses is limited. In this study, the miRNA expression patterns in maize in response
to E. turcicum stress were investigated using a plant miRNA microarray platform. A total of 118
miRNAs were detected in mock- and E. turcicum-inoculated leaves. Among these miRNAs, miR530,
miR811, miR829, and miR845 were identified as new miRNAs in maize through a homology-based
approach. In addition, four miRNAs were differentially regulated in response to E. turcicum: miR811,
miR829, miR845, and miR408.
Key words: ; miRNA; Microarray; Differential expression; Maize
0 Introduction
Maize is one of the most important staple food crops in the world. Maize diseases are a major
limiting factor in its production. Among these diseases, Turcicum blight, caused by Exsero

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