文档介绍：:..牛肉中阿莫西林残留的高效液相色谱分析摘要:建立了反高效液相色谱分析牛肉样品中阿莫西林残留量的方法。对动物组织样品采用磷酸盐缓冲溶液提取、三氯乙酸沉淀蛋白、固相萃取净化和富集。研究了样品在C18柱上的保留行为和阿莫西林的特征谱图。待测物用高效液相色谱法分离,外标定法定量分析,标准曲线的线性良好,,〜500ug/%〜%,%〜%,最低检出限低于10ug/kg。该分析方法具有良好的重现性。关键词:髙效液相色谱;固相萃取;阿莫西林;残留量;牛肉DeterminationofAmoxicillinResidueinBeevesbyHighPerformanceLiquidChromatographyAbstract:Areversed-phasehighperformanceliquidchromatographicmethod(RP-HPLC)hasbeendevelopedforthedeterminationofamoxicillinresidueinbeeves・Beeveswereextractedusingphosphatebuffer,precipitatedproteinswithtrichloroaceticacid(TCA)solution,cleanedupwithandenrichedbysolidphaseextraction(SPE).-()-500(ig/%%,%%・Thelimitofdetectionwasbelow10ug/kg<Therelativestandarddeviationsofintraassayandinter-assaywerebothwithin10%・Thisanalysismethodhasgoodrecurrence・Keyword:HPLC;solidphaseextraction;amoxicillin;residue;Beeves阿莫西林<amoxicillin儿化学名称为羟氨节青霉素,属于青霉