文档介绍：实验二细菌生理及外界因素对细菌的影响
示教
细菌的生长现象
液体、固体、半固体培养基
生物因素对细菌的影响
药敏实验
噬菌体对细菌的作用
细菌的代谢产物的检查
乳糖发酵实验
硫化氢实验
尿素分解实验
操作
紫外杀菌实验
( 大肠埃希菌、枯草杆菌)
细菌分离培养方法——平板划线法
(混合菌液)
细菌代谢产物检查方法——IMViC试验接种
(大肠埃希菌、产气肠杆菌)
Nutrient requirements of bacterial growth
water
Carbon source
Nitrogen source
Minerals
Growth factors:
pounds such as amino acids, purines嘌呤, pyrimidines嘧啶, and vitamins that a cell must have for growth but cannot synthesize itself.
culture medium培养基: Substances used for artificial cultivation of bacteria.
Classification of culture medium:
According to basic ingredients:
Minimal essential growth medium基础培养基
enrichment medium增菌培养基
selective medium选择培养基
differential medium鉴别培养基
According to physical condition:
liquid medium液体培养基
solid medium固体培养基: ~% of agar
semisolid medium半固体培养基: ~% of agar
Phenomena of bacterial growth 细菌在培养基中的生长情况
In liquid medium: surface growth pellicle表面生长, uniformly turbid均匀混浊生长, sediment in bottom沉淀生长
On solid medium :
In semisolid medium :
无动力有动力
Ⅳ
红
青
链
抑菌圈
susceptibility test 药敏试验
1
2
3
涂布痢疾杆菌
涂布大肠杆菌
肉汤
痢疾杆菌噬菌体
接种大肠杆菌
接种枯草杆菌
班级、姓名/学号、大肠枯草
uv30’
培养箱