文档介绍：
拟南芥抗旱突变体的筛选鉴定及其基因
克隆#
柏晓娅,程丹,顾菊,陈光朗,孙成磊,阳立波,曹树青**
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(合肥工业大学生物与食品工程学院,合肥 230009)
摘要:干旱是植物生长和发育的限制因素之一,因此抗旱基因克隆研究非常必要。为了得到
抗旱基因,利用甘露醇模拟干旱胁迫,以发芽为筛选指标从拟南芥突变体库中筛选获得抗旱
突变体 csm1-1。生理生化分析表明,csm1-1 抗旱性与其体内电导率变化、可溶性糖含量升
高和脯氨酸积累有关。利用 TAIL-PCR 技术和 RT-PCR 分析,发现 CSM1 基因(RCI3)编
码一个过氧化物酶。以上发现为进一步研究该基因调控植物抗旱的机理提供了基础。
关键词:抗旱;拟南芥;筛选;鉴定;TAIL-PCR
中图分类号:Q754
Identification of the Arabidopsis Drought-Tolerant Mutant
and its Gene Cloning
BAI Xiaoya, CHENG Dan, GU Ju, CHEN Guanglang, SUN Chenglei, YANG Libo,
CAO Shuqing
(School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009)
Abstract: Drought is one of the limiting factors for plant growth and development, and therefore
cloning of novel drought-tolerance genes is very necessary. For obtaining the gene of
drought-tolerant ,The wild-type and mutant lines of Arabidopsis were treated with mannitol to
simulate drought stress to screen for drought -resistant mutants by germination analysis. One
drought-tolerant csm1-1 mutant was isolated. Physiological and biochemical analysis showed that
enhanced drought tolerance of csm1-1 was consistent with lower conductivity,and increased
accumulations of soluble sugars and proline acid. Using TAIL-PCR and RT-PCR, we found that
CSM1 is the RCI3 gene encoding a peroxidase. These results provide basis for further research on
the