文档介绍：
RP-HPLC 测定白屈菜中白屈菜碱的含量#
曲中原1,邹翔2,季宇彬3*
(1. 哈尔滨商业大学药学院,哈尔滨
150076;
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2. 哈尔滨商业大学药物研究所博士后科研工作站,哈尔滨 150076;
3. 哈尔滨商业大学抗肿瘤天然药物教育部工程研究中心,哈尔滨 150076)
摘要:目的: 建立用高效液相色谱法测定白屈菜药材中白屈菜碱含量的方法。方法:采用甲
醇提取,高效液相色谱法测定,以乙腈-1%三乙胺溶液(20:80)(三乙胺用磷酸调节 PH 至
)为流动相,流速为 1 mL/min,检测波长为 290nm。结果:白屈菜中白屈菜碱的含量为
%。白屈菜碱在 ~ 范围内呈良好线性关系,r=(n=6),回收率为
±%,RSD 为 %。结论:该方法简便快速,结果准确可靠,可作为白屈菜药材
及其制剂质量控制方法之一。
关键词:中药学;白屈菜;白屈菜碱;高效液相色谱法
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中图分类号:R282
Determination of Chelidonine content in Chelidonium majus
by RP-HPLC
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QU Zhongyuan1, Zou Xiang2, Ji Yubin3
(1. Pharmaceutical School, Harbin University merce, Harbin 150076;
2. Postdoctoral Programme of Pharmacology Institute, Harbin University merce, Harbin
150076;
3. Engineering Research Center of Natural Anticancer Drugs, Harbin University merce,
Harbin 150076)
Abstract: Objective: To establish the method for determination of chelidonine in Chelidonium
majus. Methods: The fine powder of the Chelidonium majus was extracted with methanol and the
content of chelidonine was determined by mobile phase was acetonitrile-1%
triethylamine (20∶80) ( adjusted pH to 3 using phosphoric acid) and the detection wavelength
was set at 290nm. Results: The chelidonine content in Chelidonium majus was %. The linear
range of chelidonine was ~(r=, n=6), the average recovery was