文档介绍：RP-HPLC法测定平消片中马钱子碱和士的宁的含量
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【摘要】目的采用反相高效液相色谱法(RP-HPLC)测定平消片中马钱子碱和士的宁的含量。方法采用lichrospher C18色谱柱( mm×250 mm,5 μm);流动相为水-乙腈(60∶40),每1 000 g、 g; mL/min;检测波长254 nm;柱温25 ℃。~ μg、~ μg范围线性关系良好, 9;%(RSD=%)、%(RSD=%)。结论本方法操作可靠、精确,适用于平消片中马钱子碱和士的宁的含量测定。
【关键词】平消片;马钱子碱;士的宁;高效液相色谱法
Abstract:Objective To establish the RP-HPLC method for determination of Bruchine and Strychine in Pingxiao Tablet. Methods The lichrospher C18 ( mm×250 mm, 5 μm) was Used as the stationary phase. The Mobile phase consisted of water-acetonitrile (60∶40) with g sodium dodecylsulphate and g KH2PO4 in 1 000 mL mixture. The
Detecting wavelength was 254 nm and flow rate was mL/min. The column temperature was 25 ℃. Result The linear ranage of Bruchine and Strychine were ~ μg and ~ μg respectively. The recovery rates of bruchine and strychnine were % (RSD=%) and % (RSD=%) respectively. Conclusion This method is realiable and accurate, the method can be applied to determination the contents of Bruchine and Strychine in Pingxiao Tablet.
Key words:Pingxiao Tablet;Bruchine;Strychine;HPLC
平消片主要由郁金、枳壳(麸炒)、仙鹤草、五灵脂、马钱子粉、硝石、干漆、白矾8味药组成,具有行气散瘀、止痛散结、扶正祛邪的功能,有缓解肿瘤患者症状、缩小瘤体、提高免疫力的功效[1]。马钱子粉为该方的主药,它主要含有马钱子碱(Bruchine)、士的宁(Str