水稻苗期耐冷相关QTL qCTS3-1的鉴定和分子定位.doc

文档介绍：
水稻苗期耐冷相关 QTL qCTS3-1 的鉴定和
分子定位#
周勇,王中德,陶亚军,缪军,高云,魏伟,梁国华**
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(扬州大学农学院,江苏扬州 225009)
摘要:本研究以广陆矮 4 号为受体,日本晴为供体的染色体片段代换系群体中的 1 个低温敏
感株系 C32022 为材料,以低温处理后植株存活率为指标,采用代换作图法在水稻第 3 染
色体上定位了一个控制水稻苗期耐冷性的 QTL,命名为 qCTS3-1。利用 C32022 和广陆矮 4
号衍生的 F2 分离群体进行遗传分析,结果表明,该群体中叶片表现正常绿色和叶片表现出
黄色、萎蔫单株比例符合 1 : 3 的分离比,表明耐冷性受单个主效 QTL 控制,且低温敏感对
低温耐受为显性来自日本晴的等位基因增加对低温的敏感性。利用该群体中 86 个隐性单株
将 qCTS3-1 初步定位在区 WZD-S3-7~WZD-S3-11 之间,物理距离约为 550kb。研究结果为
qCTS3-1 的精细定位和克隆奠定了基础。
关键词:水稻;耐冷性;数量性状位点;分子定位
中图分类号:S511
Identification and Mapping of a Major QTL qCTS3-1
Controlling Cold Tolerance at Seeding Stage in Rice
ZHOU Yong, WANG Zhongde, TAO Yajun, MIAO Jun, GAO Yun, WEI Wei,
LIANG Guohua
(College of Agriculture, Yangzhou University, JiangSu YangZhou 225009)
Abstract: In this study, we characterized a cold tolerance sensitive line C32022 from a set of
chromosome segment substitution lines derived from a cross between Guangluai 4 as the recurrent
parent and Nipponbare as the donor. Survival rate after cold treatment was selected as the
screening index for cold tolerance at seedling stage. A major QTL, qCTS3-1, was mapped on
chromosome 3 using substitution mapping strategy. In an F2 segregating population consisting of
401 plants derived from C32022 and Guangluai 4, the frequency distribution of qCTS3-1 was
shown to be an expected Mendelian ratio for single locus seg