文档介绍：
产紫杉醇真菌 NK-102 发酵条件的优化#
娄静,陈龙飞,于溪,郝晓冉,朱旭东,潘皎**
(南开大学生命科学学院,天津 300071)
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摘要:NK-102 是本实验室分离得到的一株产紫杉醇的球毛壳真菌。本研究结合单因子试验
和正交试验对 NK-102 的液体发酵培养基和发酵条件进行优化。单因子试验表明,以可溶性
淀粉作为碳源,蛋白胨作为氮源明显优于其他碳氮源;进而通过正交试验得出最佳的摇瓶培
养基配方及培养温度为:简易马铃薯培养基中添加 %淀粉、4%蛋白胨,培养温度 26℃;
优化后的最佳发酵条件:摇瓶转速 180rpm、摇瓶装量 20%、最佳 pH 缓冲剂 75 mM MES-Tris。
优化后的发酵生物量干重提高了 %,此研究结果为实现该菌株工业化发酵生产紫杉醇
提供了科学基础。
关键词:球毛壳属;发酵;正交试验;紫杉醇
中图分类号:Q78;
Optimize the fermentation conditions of taxol producing
fungus NK-102
LOU Jing, CHEN Longfei, YU Xi, HAO Xiaoran, ZHU Xudong, PAN Jiao
(College of Life Sciences, Nankai University, TianJin 300071)
Abstract: Our lab has isolated a taxol producing chaetomium fungus named NK- this study
the liquid fermentation medium and fermentation conditions of NK-102 were optimized by single
factor and orthogonal experiments . Single factor experiments showed that soluble starch as a
carbon source, peptone as a nitrogen source were better than other carbon and nitrogen sources.
Through orthogonal tests,we got the optimum medium formula and best culture temperature:
simple potato medium supplemented with % starch, 4% peptone, culture temperature 26 ℃;
The optimal fermentation conditions: shaking speed 180rpm, bottle filled with 20% of the medium,
the optimum pH buffer is 75 mM MES-Tris. The dry weight of the fungus increased % after
using the opt