LRRC4作用Hedgehog信号通路抑制脑胶质瘤细胞增殖.doc

文档介绍：
LRRC4 作用 Hedgehog 信号通路抑制脑胶
质瘤细胞增殖#
窦志金,刘幸,杜文众,孙颖,蒋传路**
5
10
(哈尔滨医科大学附属第二医院神经外科)
摘要:目的探讨 LRRC4(leucine-rich repeats containing 4)通过 Hedgehog 信号通路对脑胶
质瘤细胞增殖的影响。方法将携带外源性 LRRC4 基因的质粒分别转入脑胶质瘤细胞 U251
和 U87 细胞中,通过 qRT-PCR 和 Western-blot 方法检测 LRRC4 对 Hedgehog 信号通路中的
Smo、GLI1 及其下游靶基因(N-myc、CyclinD1、Foxm1)表达的影响;MTT 实验、细胞
集落形成实验检测分析 LRRC4 对 U251 和 U87 细胞增殖的影响。流式细胞分析 LRRC4 对
U87 细胞周期的影响。结果
过表达 LRRC4 可以下调 Smo 和 GLI1 及其下游 N-myc、CyclinD1
和 Foxm1 的核酸及蛋白水平;过表达 LRRC4 能抑制胶质瘤细胞 U87 及 U251 的增殖并使细
胞周期 G0/G1 期阻滞。结论过表达 LRRC4 可作用 Hedgehog 信号通路抑制胶质瘤细胞的增
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殖。。
关键词:脑胶质瘤;Hedgehog 通路;LRRC4;增殖
中图分类号:
Inhibitory effect of LRRC4 on the proliferation of glioma
cells through Hedgehog signaling pathway
Dou Zhijin, Liu Xing, Du Wenzhong, Sun Ying, Jiang Chuanlu
(The Second Affiliated Hospital of Harbin Medical University,Harbin,Heilongjiang 150086)
Abstract: 【Objective】To explore the effect of LRRC4 on the proliferation of glioma cells by the
Hedgehog signaling pathway.【Methods】LRRC4 was transfected into U251 and U87 glioma cells.
RT-PCR and Western-blot were used to identify the role of LRRC4 on regulating the expression
of the key molecules(Smo,GLI1) in Hedgehog signaling pathway and its downstream target
molecules (N-myc,CyclinD1,Foxm1). MTT assay and Colony formation assay were performed to
determine the effect of LRRC4 on U251 and U87 cells prolife