文档介绍：
ChIP-chip 技术在肿瘤研究中的应用进展#
朱新江,戴冬秋**
(中国医科大学附属四院胃肠外科,沈阳 110032)
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摘要:肿瘤的发生、发展是一个复杂的连续过程, 众多基因或蛋白表达产物在不同时段异常
表达以及它们之间构成的复杂调控网络使得肿瘤的生物学行为至今仍未得以阐明。生物芯片
是后基因组时代肿瘤学研究中的重要手段,其中 Chip 技术与芯片技术相结合( ChIP-chip)的
出现,成为研究一些关键组分活性的有力武器, 广泛用于肿瘤细胞的表遗传修饰、DNA-蛋
白结合位点的研究。通过结合位点分析信息与基因表达数据相联系, 将有助于寻找生物标志
物, 研究疾病的分子病理学等, 从而了解基因表达的机制, 找到调控基因表达的方法, 研制
出有效的治疗手段。本文重点对 ChIP-chip 技术在肿瘤研究和诊断治疗中的应用做一简要综
述。
关键词:ChIP-chip;肿瘤;基因表达;表遗传学
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Advances of

ChIP-chip in cancer research
ZHU Xinjiang, DAI Dongqiu
(Department of Gastrointestinal Surgery,the Fourth Affiliated Hospital,China Medical University,
Shenyang 110032)
Abstract: The development of cancer is a process plex and consecutive changes, Many
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abnormal gene or the product of protein expression in different times and tumor biological
behavior has yet to be elucidated. Biochip is an important technique in the post genome era for
oncology research, The Chip technology and bination of chip technology (Chip - chip),
e the powerful weapon of the some of the ponents of activity, Chip-chip had widely
used in the field of the tumor cells ic modification and DNA - protein binding site.
Through the analysis of the binding site information associated with gene expression data,which
will help to find biomarkers, research on molecular pathology of the disease,and so on. To
understanding the mechanisms of gene expressi