文档介绍：
发根农杆菌诱导的决明毛状根与实生根的
解剖结构比较#
谭燕,米瑶,黄靖,朱林慧,李关荣**
5
10
15
20
25
30
35
40
(西南大学农学与生物科技学院,重庆 400716)
摘要:目的:找出发根农杆菌诱导的决明毛状根与其无菌实生根的形态和解剖特征差异。方
法:采用体视显微观察、石蜡切片,番红-固绿染色及多糖-高碘酸-席夫反应染色,显微观察
比较决明毛状根与实生根的形态、解剖结构及淀粉粒分布。结果:决明毛状根粗细均匀,根
尖较实生根粗壮,有较多根毛;毛状根表皮细胞较小、排列较紧密,较不规则;皮层细胞排
列紧密不整齐;内皮层细胞排列紧密,细胞间隙小,凯氏带不明显;毛状根初生木质部为二
原型,而实生根为四原型;毛状根形成层细胞较实生根细胞小、排列紧密、层数多;毛状根
根冠几乎没有淀粉粒,而实生根有较多淀粉粒。结论:决明毛状根与实生根无论在外部形态
还是在解剖结构上均存在较大差异。这为毛状根的细胞发生机制、生理生化和基因表达特性
以及其无限非向地性生长分子机制的进一步研究奠定了很好的基础。
关键词:决明;毛状根;实生根;解剖结构比较
parison of the hairy roots induced by
Agrobacterium
rhizogenes and the seedling roots of Senna obtusifolia L
TAN Yan, MI Yao, HUANG Jing, ZHU Linhui, LI Guanrong
(College of Agronomy and Biotechnology, Southwest University, ChongQing 400716)
Abstract: Objective: to identify the morphological and anatomical differences between the
Agrobacterium rhizogenes induced hairy roots and the aseptic seedling roots of Senna obtusifolia
L. Methods: stereomicroscopic and light microscopic observation parison of morphology,
the paraffin sections stained with safranine and fast green and haride-periodate-Schiff
(PAS) reaction to identify the differences in morphology, anatomy and the distribution of starch
granules of the hairy roots and the seedling roots. Results: the hairy roots were more uniform in
thickness, their root-tips were thicker and more robust, and had more root hairs than