供肝缺血与再灌注损伤的蛋白质组学对照研究.doc

文档介绍：
供肝缺血与再灌注损伤的蛋白质组学对照
研究#
吴斌,陆敏强**
5
10
(中山大学附属第三医院肝移植中心,广州 510630)
摘要: 目的:研究肝移植手术人体供肝组织缺血-再灌注损伤的相关蛋白质变化规律。方法:
2009 年 3 月,对 3 例肝移植供肝于以下三个时间点切取肝组织标本进行对照研究,①新鲜
的供肝组织。②经过缺血降温保存后修整完毕,但未经血管吻合的供肝组织。③复流后的供
肝组织。通过 2DE-MALDI-TOF 和质谱技术,对上述三个时间点的蛋白表达作出比较。结
果:共分离出 1580 个蛋白点,筛查并鉴定出与缺血再灌注损伤有关的蛋白 19 个,结合 19
个蛋白质的功能,对这些蛋白质的变化特征进行分析。结论:供肝缺血性损伤和再灌注损伤
涉及的蛋白质种类不同,各种蛋白质在不同损伤阶段有不同的变化方式。
关键词:肝移植;缺血再灌注损伤;蛋白质组学
中图分类号:R617
15
Comparative proteomic analysis of human donor liver
subjected to ischemia injury with reperfusion injury
WU Bin, LU Minqiang
(Department of liver transplantation, the 3rd affiliated hospital of Sun-Yat-Sen University,
20
25
30
35
40
GuangZhou 510630)
Abstract: Objective:To evaluate the ischemia and reperfusion injury associated proteomics of
human donor liver during liver transplantation (LT). Methods:At Mar 2009, we studied 9 sample
liver tissue, 3 obtained from each donor liver at three different time points. The three time points
represent normal control, ischemia injury, and reperfusion injury respectively. By using method of
2DE-MALDI-TOF and mass-spectrum, the differential expression of these protein at T1,T2,and
T3 time point pared. Results:From the 1580 proteins that were isolated, 19 IRI-related
proteins varied markedly from time point T1to T3 and were identified. The character of each
protein was further analyzed based on their functions