亚精胺诱导宫颈癌Hela细胞活力变化研究.doc

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亚精胺诱导宫颈癌 Hela 细胞活力变化研究#
张军,漆永梅,古雪岩,田一虹,黄德军,张迎梅**
(兰州大学生命科学学院,兰州 730000)
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摘要:亚精胺(Spermidine)是细胞内一种重要的多胺物质,其在分解代谢过程中会生成过
量的 ROS 等代谢产物,并影响细胞活力,然而亚精胺导致人宫颈癌 Hela 细胞活力下降机理
尚未见报道。本研究选取 Hela 细胞为实验材料,采用亚精胺处理,开展了以下研究;(1)
20 µM 亚精胺连续处理 0-24 h,1 h 时细胞内亚精胺含量即达到峰值,且为对照组的 3 倍,
说明亚精胺可在短时间内大量进入细胞;(2)0-125 µM 亚精胺处理 24 h 后,细胞活力显著
(≦50 µM)或极显著(≧ 75µM)下降,具有明显的浓度效应;(3)60 µM 亚精胺处理 6
h 后,细胞内谷胱甘肽(GSH)含量是对照组的 16%左右,而氮-乙酰半胱氨酸(NAC)或
GSH 和亚精胺联合处理细胞时,与亚精胺单独处理组相比,细胞活力显著升高。研究结果
表明,亚精胺可能主要通过耗竭细胞内 GSH 导致细胞活力下降。
关键词:毒理学;亚精胺;谷胱甘肽;氮-乙酰半胱氨酸;细胞活力
中图分类号:R99
The study of spermidine on cell viability of Hela cell
Zhang Jun, Qi Yongmei, Gu Xueyan, Tian Yihong, Huang Dejun, Zhang Yingmei
(School of Life Science, Lanzhou University, LanZhou 730000)
Abstract: Spermidine, an important intracellular polyamine, can produce reactive oxygen species
(ROS) during its catabolism. It is well accepted that excessive ROS can affect cell viability.
However, there is no report about why and how spermidine declines Hela cell viability. The
present research selected Hela cells as experimental material, and treated the cells with spermidine.
The following researches were done: (1) Hela cells were treated with 20 μM spermidine for 0-24 h.
The results of HPLC showed that the intracellular content of spermidine was about 3 times higher
than that of the control group after 1 h treatment