罗丹明B类荧光探针的合成及其对铜离子的检测.doc

文档介绍：
罗丹明 B 类荧光探针的合成及其对铜离子
的检测
魏荣严,赵峰,严世强**
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(兰州大学化学化工学院,兰州 730000)
摘要:以罗丹明 B 和乙酰氯为原料,合成了罗丹明 B 类 Cu2+荧光增强型分子探针 RAC。在
乙腈/水(1:9,v/v,pH=)溶液中,该探针本身无色,加入 Cu2+后很快变为桃红色,荧光强
度明显增强,而其它常见金属离子如 Ag+, Ca2+, Hg2+, Fe3+, Mg2+, Cd2+, Co2+, Ni2+, K+, Zn2+,
Pb2+和 Na+不引起或引起很小的紫外-可见或荧光光谱变化。该探针与 Cu2+的结合比例为 1:1,
探针能选择性的检测 Cu2+,且检出限浓度可达到 10-6molL-1,可用于环境中铜离子的检测。
关键词:荧光探针;铜离子;罗丹明 B;乙酰氯
中图分类号:O069
Synthesis of fluorescent probe based on rhodamine B and its
appalication in the determination for Cu2+
Wei Rongyan, Zhao Feng, Yan Shiqiang
(College of Chemistry and Chemical Engineering,Lanzhou University, LanZhou 730000)
Abstract: A new fluorescence-enhanced Cu2+ probe RAC based on rhodamine B and Acetyl
Chloride was synthesized. It was found that the fluorescence intensity of RAC increased with the
addition of Cu2+ in acetonitrile/water(1:9, v/v, pH=)solution. The addition of Cu2+ ion led to
obvious color change from colorless to pink as well as significantly enhanced fluorescence, while
mon metal ions such as Ag+, Ca2+, Hg2+, Fe3+, Mg2+, Cd2+, Co2+, Ni2+, K+, Zn2+, Pb2+
and Na+ induced no or much smaller color/fluorescent spectral changes. The selectivity and
sensitivity of RAC were very high. RAC formed 1:plex with Cu2+, and has the detection
limit of 10-6 molL-1 which can be used in the detection of Cu2+ in environment.
Keywords: luorescence probe; Cu2+;