文档介绍：鬼臼毒衍生物诱导人肺腺癌A549细胞凋亡(医学论文)鬼臼毒衍生物诱导人肺腺癌A549细胞凋亡【关键词】鬼臼***,肺肿瘤,腺癌,,细胞凋亡,抗原,CD95,基因,BclApoptosisinhumanlungadenocarcinomacelllineA549byderivativeofpodophyllotoxin【Abstract】AIM:2,aderivativeofpodophyllotoxin,:MTTassay,electronmicroscopy,,,48,,,,;agarosegelelectrophoresisshowedanevidentDNAfragmentation;cellcycleanalysis更多精品文档,欢迎来我主页查询indicatedanincreasedSphaseproportion,aswellasanapparentS/2decreasedBcl2proteinexpression(P<),whichisprobablyassociatedwiththedecreasedBcl2expressionandcellcyclearrest.【Keywords】podophyllotoxin;lungneoplasms;adenocarcinoma;apoptosis;antigens,CD95;genes,Bcl2【摘要】目的:2对人肺腺癌细胞A549的凋亡诱导作用,:采用四***偶氮唑蓝(MTT)比色法、电子显微镜、DNA琼脂糖凝胶电泳和细胞周期分析法检测A549细胞凋亡,采用流式细胞术(FCM)测定细胞Bcl2,,24,48,,,,电镜观察呈现典型的凋亡形态改变,DNA电泳可见明显的梯状条带,细胞周期分析显示S期细胞数明显增多,出现S/2能显著降低Bcl2蛋白表