文档介绍：August 13, 1996 8:44 Annual Reviews Chapter15 AR015-Ch15
Annu. Rev. Microbiol. 1996. 50:467–90
Copyright ?c 1996 by Annual Reviews Inc. All rights reserved
ICALLY ENGINEERED
SYNTHESIS OF NATURAL
PRODUCTS: From Alkaloids to Corrins
Charles A. Roessner and A. Ian Scott
Center for Biological Nuclear ic Resonance, Department of Chemistry, Texas
A&M University, College Station, Texas, 77843-3255
KEY WORDS: binant enzymes, in vitro multienzyme synthesis, carbohydrate synthesis,
polyketide synthesis, vitamin B12 synthesis
ABSTRACT
Because many natural products are of biological and medicinal importance, meth-
ods are continually being sought for studying their biosynthetic pathways, which
may eventually result in increased production and the generation of -
pounds. Advances in ic engineering have enabled the homologous or het-
erologous expression of many natural product biosynthetic genes from divergent
sources, resulting in a supply of enzymes not readily available by isolation from
the anism. Mixing and matching of these enzymes in cell-free re-
actions can provide information, not available by any other means, about enzyme
mechanisms, pathway intermediates, and possible variations in the structure of
the final product.
CONTENTS
INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 468
COFACTOR REGENERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 469
Nucleoside Triphosphates (NTPs) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 470
Nicotinamide Cofactors [NAD(P)(H)] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 470
Acyl Coenzyme A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 470
S-Adenosyl Methionine (SAM). . . . . . . . . . . . . . . . . . . .