文档介绍：β-1,4-葡聚糖硫酸酯的制备及其抗凝血活性
王兆梅,李琳,郭祀远,肖凯军
(华南理工大学轻化工研究所,广东广州510640)
摘要: 对微晶纤维素(MCC)进行硫酸酯化修饰,得到多种β-1,4-葡聚糖硫酸酯(GS),其硫酸取代度(Ds)~。。IR分析表明,MCC通过硫酸化反应引入了硫酸酯基13C NMR揭示,硫酸酯化主要发生在C6,部分在C2,而C3位不发生硫酸酯化反应。凝血分析表明,(tAPTT)和凝血酶时间(tTT),使血浆tAPTT延长两倍,,低于活性为150IU/mg的肝素,在一定质量浓度范围内,GS的体外抗凝血活性与肝素相当。显色分析揭示,GS的抗凝血机制主要在于通过抗凝血酶AT-Ⅲ的调节作用抑制凝血因子Ⅱa和Xa的活性。
关键词: 葡聚糖硫酸酯;抗凝血;机制
中图分类号:TQ35;O614 文献标识码:A 文章编号:0253-2417(2006)04-0001-05
Preparation and Anticoagulation Activity of β­1,4­Glucan Sulfate
WANG Zhao­mei, LI Lin, GUO Si­yuan, XIAO Kai­jun
(Research Institute of Light Industry and Chemical Engineering,South China University of Technolage, Guangzhou 510640, China)
Abstract: Various β­1,4­glucan sulfates(GS) were synthesized by sulfate of microcrystalline cellulose. Their sulfate substitute degree(Ds) varied in the range of to with Ds of was selected for structural analysis and anticoagulation spectra showed the introduction of sulfate groups by NMR indicated that sulfation occurred mainly at C6, partially at C2 and no sulfate substitution occurred at C3. Coagulation assays showed that mg/L GS could significantly prolong tAPTT and tTT in dosage of GS required to double tAPTT of normal human plasma was mg/L, lower than that of heparin with the anticoagulation activity of 150 IU/mg. Its potent of anticoagulation in vitro reached the efficacy of heparin in definite range of concentration. Chromogenic analysis revealed that the anticoagulation mechanism of GS was mainly due to the inhibition of the coagulation factors Ⅱa & Xa activities mediated by antithrombin AT­Ⅲ.
Key words: glucan sulfate;anticoagulation;mechanism
E1级胶合板用脲醛树脂的13C NMR定量分析
金立维,王春鹏,周道兵,储富祥*,赵临五
(中国林业科学研究院林产化学工业研究所;国家林业局林产化学工程重点开放性实验室,江苏南京210042)
摘要: 采用13C NMR定量分析了由选定碱-酸-碱工艺所合成的E1级胶合板用脲醛树脂。结果表明: %的甲醛分子转化成了羟甲基脲, %的甲醛分子转化成了亚甲基醚键。在随后的酸性缩聚阶段中,64 %的羟甲基脲发生